Immunohistochemical Approach Reveals Involvement Of Inducible Nitric Oxide Synthase In Rat Late Development.

dc.creatorZambuzzi, W F
dc.creatorPaiva, K B S
dc.creatorBatista, A C
dc.creatorLara, V S
dc.creatorGranjeiro, J M
dc.date2009-Jun
dc.date2015-11-27T13:15:24Z
dc.date2015-11-27T13:15:24Z
dc.date.accessioned2018-03-29T01:09:08Z
dc.date.available2018-03-29T01:09:08Z
dc.identifierJournal Of Molecular Histology. v. 40, n. 3, p. 235-40, 2009-Jun.
dc.identifier1567-2387
dc.identifier10.1007/s10735-009-9225-2
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19588257
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198399
dc.identifier19588257
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298632
dc.descriptionTo better understand the role of nitric oxide (NO) in mammal development, specifically in the transition of the fetal stages at birth, we studied the timing of cell-specific expression of inducible NO synthase (iNOS) isoform during gestational periods of rats, mainly at the late stages of intra-uterine development. Before experimentation, the samples were collected (from 17th to 21st gestational days), fixed in 10% buffered formalin and embedded in paraffin for histological procedures. Hereafter, the sections (5 mum thickness) obtained from different embryos were immunostained by avidin-biotin-immunoperoxidase technique, by using antibody against iNOS isoform. The most of cell immunopositive was suggestive of granulocyte-like cells and those cells were resident close to the blood vessels in different organs, such as: lung, liver or bone marrow environment. Sometimes we noted immunopositive cells in the blood flow, as reported in the thymus. In agreement, iNOS expression, obtained by western blotting analysis, showed the same profile. Together, our data shows that iNOS expression increased gradually during the late stages of rat development (from E17 to E21) and it was executed by cells close to blood vessels. Thus, we can clearly to predict that this expression was finely modulated and it contributes for time-line dependent NO production during rat late development.
dc.description40
dc.description235-40
dc.languageeng
dc.relationJournal Of Molecular Histology
dc.relationJ. Mol. Histol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectEmbryo, Mammalian
dc.subjectEmbryonic Development
dc.subjectFemale
dc.subjectGene Expression Regulation, Developmental
dc.subjectGene Expression Regulation, Enzymologic
dc.subjectImmunohistochemistry
dc.subjectNitric Oxide Synthase Type Ii
dc.subjectRats
dc.subjectRats, Wistar
dc.titleImmunohistochemical Approach Reveals Involvement Of Inducible Nitric Oxide Synthase In Rat Late Development.
dc.typeArtículos de revistas


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