Nitrite Reduction And Superoxide-dependent Nitric Oxide Degradation By Arabidopsis Mitochondria: Influence Of External Nad(p)h Dehydrogenases And Alternative Oxidase In The Control Of Nitric Oxide Levels.

dc.creatorWulff, Alfredo
dc.creatorOliveira, Halley Caixeta
dc.creatorSaviani, Elzira Elisabeth
dc.creatorSalgado, Ione
dc.date2009-Sep
dc.date2015-11-27T13:15:23Z
dc.date2015-11-27T13:15:23Z
dc.date.accessioned2018-03-29T01:09:06Z
dc.date.available2018-03-29T01:09:06Z
dc.identifierNitric Oxide : Biology And Chemistry / Official Journal Of The Nitric Oxide Society. v. 21, n. 2, p. 132-9, 2009-Sep.
dc.identifier1089-8611
dc.identifier10.1016/j.niox.2009.06.003
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19576290
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198390
dc.identifier19576290
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298623
dc.descriptionMitochondria recently have emerged as important sites in controlling NO levels within the cell. In this study, the synthesis of nitric oxide (NO) from nitrite and its degradation by mitochondria isolated from Arabidopsis thaliana were examined. Oxygen and NO concentrations in the reaction medium were measured with specific electrodes. Nitrite inhibited the respiration of isolated A. thaliana mitochondria, in competition with oxygen, an effect that was abolished or potentiated when electron flow occurred via alternative oxidase (AOX) or cytochrome c oxidase (COX), respectively. The production of NO from nitrite was detected electrochemically only under anaerobiosis because of a superoxide-dependent process of NO degradation. Electron leakage from external NAD(P)H dehydrogenases contributed the most to NO degradation as higher rates of Amplex Red-detected H(2)O(2) production and NO consumption were observed in NAD(P)H-energized mitochondria. Conversely, the NO-insensitive AOX diminished electron leakage from the respiratory chain, allowing the increase of NO half-life without interrupting oxygen consumption. These results show that the accumulation of nitric oxide derived from nitrite reduction and the superoxide-dependent mechanism of NO degradation in isolated A. thaliana mitochondria are influenced by the external NAD(P)H dehydrogenases and AOX, revealing a role for these alternative proteins of the mitochondrial respiratory chain in the control of NO levels in plant cells.
dc.description21
dc.description132-9
dc.languageeng
dc.relationNitric Oxide : Biology And Chemistry / Official Journal Of The Nitric Oxide Society
dc.relationNitric Oxide
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectArabidopsis
dc.subjectArabidopsis Proteins
dc.subjectData Interpretation, Statistical
dc.subjectElectron Transport Complex Iii
dc.subjectElectron Transport Complex Iv
dc.subjectMitochondria
dc.subjectMitochondrial Proteins
dc.subjectNad
dc.subjectNadh Dehydrogenase
dc.subjectNitric Oxide
dc.subjectNitrites
dc.subjectOxidoreductases
dc.subjectOxygen
dc.subjectPlant Proteins
dc.subjectSuperoxides
dc.titleNitrite Reduction And Superoxide-dependent Nitric Oxide Degradation By Arabidopsis Mitochondria: Influence Of External Nad(p)h Dehydrogenases And Alternative Oxidase In The Control Of Nitric Oxide Levels.
dc.typeArtículos de revistas


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