Structural Characteristics Of The Tendinous Cord-papillary Muscle Junction In Healthy And Hypertensive Rats.

dc.creatorFrancia-Farje, Luis Alberto Domingo
dc.creatorAlmeida-Francia, Camila Contin Diniz
dc.creatorMatheus, Selma Maria Michelin
dc.creatorTorrejais, Marcia Miranda
dc.creatorSoares, Jair de Campos
dc.date2009-Oct
dc.date2015-11-27T13:15:21Z
dc.date2015-11-27T13:15:21Z
dc.date.accessioned2018-03-29T01:09:04Z
dc.date.available2018-03-29T01:09:04Z
dc.identifierMicron (oxford, England : 1993). v. 40, n. 7, p. 691-701, 2009-Oct.
dc.identifier1878-4291
dc.identifier10.1016/j.micron.2009.05.004
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19564120
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198383
dc.identifier19564120
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298616
dc.descriptionAlthough the myotendinous junction (MTJ) of skeletal striated muscle is well known, more detailed studies regarding the structure of the cardiac MTJ are scarce. The objective of the present study was to investigate the morphological characteristics of the MTJ in hearts of healthy and hypertensive (SHR) female rats using histological, ultrastructural and three-dimensional (SEM) methods, as well as to evaluate the expression of vinculin by immunofluorescence. In the two groups, light microscopy showed branching tendinous cords and collagen bundles penetrating the apex of the finger-like projections of the papillary muscle. SEM analysis revealed an enlarged apex of the papillary muscle in SHR which was not observed in healthy animals. The loss of force transmission appears to be compensated by the amplified connection between the papillary muscle and valvular collagen. A large number of intercalated disks close to the fiber apex, small amounts of an amorphous intercellular substance and numerous vesicles were observed in SHR. In these animals, the expression of vinculin was more marked showing a regular distribution and a pattern of transverse striations along the sarcolemma. The presence of this protein in transverse bands suggests that vinculin surrounds myofibrils in the region of the Z band. Vinculin staining was also more marked in the region of the tendinous cord-papillary muscle junctions of SHR compared to control animals. Vinculin was quantified by electrophoresis and higher amounts of this protein were observed in SHR compared to control animals.
dc.description40
dc.description691-701
dc.languageeng
dc.relationMicron (oxford, England : 1993)
dc.relationMicron
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCollagen
dc.subjectFemale
dc.subjectHypertension
dc.subjectMicroscopy, Electron
dc.subjectMicroscopy, Fluorescence
dc.subjectPapillary Muscles
dc.subjectRats
dc.subjectSarcolemma
dc.subjectTendons
dc.subjectVinculin
dc.titleStructural Characteristics Of The Tendinous Cord-papillary Muscle Junction In Healthy And Hypertensive Rats.
dc.typeArtículos de revistas


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