Organization Of Collagen Bundles During Tendon Healing In Rats Treated With L-name.

dc.creatorTomiosso, Tatiana Carla
dc.creatorNakagaki, Wilson Romero
dc.creatorGomes, Laurecir
dc.creatorHyslop, Stephen
dc.creatorPimentel, Edson Rosa
dc.date2009-Aug
dc.date2015-11-27T13:15:19Z
dc.date2015-11-27T13:15:19Z
dc.date.accessioned2018-03-29T01:09:00Z
dc.date.available2018-03-29T01:09:00Z
dc.identifierCell And Tissue Research. v. 337, n. 2, p. 235-42, 2009-Aug.
dc.identifier1432-0878
dc.identifier10.1007/s00441-009-0819-5
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19506908
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198360
dc.identifier19506908
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298593
dc.descriptionThe Achilles tendon can support high tension forces and may experience lesions. The damaged tissue does not regenerate completely, with the organization and mechanical properties of the repaired tendon being inferior to those of a healthy tendon. Nitric oxide (NO) plays an important role in wound repair. We have examined the structural reorganization and repair in Achilles tendon after injury in rats treated with the NO synthase inhibitor L-NAME. The right Achilles tendon of male Wistar rats was partially transected. One group of rats was treated with L-NAME (~300 mg/kg per day, given in drinking water) for 4 days prior to tendon sectioning and throughout the post-operative period. Control rats received water without L-NAME. The tendons were excised at 7, 14, and 21 days post-injury and used to quantify hydroxyproline and for mechanical tests. Tendons were also processed for histomorphological analysis by polarized light microscopy, which showed that the collagen fibers were disorganized by day 7 in non-treated and L-NAME-treated rats. In non-treated rats, the organization of the extracellular matrix was more homogeneous by days 14 and 21 compared with day 7, although this homogeneity was less than that in normal tendon. In contrast, in injured tendons from L-NAME-treated rats, the collagen fibers were still disorganized on day 21. Tendons from treated rats had more hydroxyproline but lower mechanical properties compared with those from non-treated rats. Thus, NO modulates tendon healing, with a reduction in NO biosynthesis delaying reorganization of the extracellular matrix, especially collagen.
dc.description337
dc.description235-42
dc.languageeng
dc.relationCell And Tissue Research
dc.relationCell Tissue Res.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAchilles Tendon
dc.subjectAnimals
dc.subjectBiomechanical Phenomena
dc.subjectCollagen
dc.subjectEnzyme Inhibitors
dc.subjectHydroxyproline
dc.subjectMale
dc.subjectNg-nitroarginine Methyl Ester
dc.subjectNitric Oxide
dc.subjectNitric Oxide Synthase
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectRegeneration
dc.subjectTendon Injuries
dc.subjectWound Healing
dc.titleOrganization Of Collagen Bundles During Tendon Healing In Rats Treated With L-name.
dc.typeArtículos de revistas


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