Fluorometric Quantification Of Green Fluorescent Protein In Tobacco Leaf Extracts.

dc.creatorRobić, Goran
dc.creatorLacorte, Cristiano
dc.creatorMiranda, Everson A
dc.date2009-Sep
dc.date2015-11-27T13:15:13Z
dc.date2015-11-27T13:15:13Z
dc.date.accessioned2018-03-29T01:08:49Z
dc.date.available2018-03-29T01:08:49Z
dc.identifierAnalytical Biochemistry. v. 392, n. 1, p. 8-11, 2009-Sep.
dc.identifier1096-0309
dc.identifier10.1016/j.ab.2009.05.016
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19457429
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198316
dc.identifier19457429
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298549
dc.descriptionThe main use of green fluorescent protein (GFP) is as a reporter system, where the existence of the protein is usually determined visually using fluorescent microscopy. Although fluorescence-based quantification of GFP is possible, background fluorescence in plants and in plant extracts was observed by our group. Another phenomenon we observed that makes quantification difficult is the increased level of GFP fluorescence in Nicotiana benthamiana leaf extracts, probably the result of dimerization of GFP molecules promoted by interaction with some component(s) of tobacco extracts. In the current work, the background fluorescence was minimized and the enhancement of GFP fluorescence in tobacco extracts was eliminated with the addition of urea to the measured solution so that a simple quantification assay for the GFP in the tobacco extracts could be established.
dc.description392
dc.description8-11
dc.languageeng
dc.relationAnalytical Biochemistry
dc.relationAnal. Biochem.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectFluorometry
dc.subjectGreen Fluorescent Proteins
dc.subjectPlant Extracts
dc.subjectPlant Leaves
dc.subjectTobacco
dc.titleFluorometric Quantification Of Green Fluorescent Protein In Tobacco Leaf Extracts.
dc.typeArtículos de revistas


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