Determination Of Short-chain Fatty Acids In Dietary Fiber Extracts Using Ion-exclusion Chromatography With Suppressed Conductivity Detection.

dc.creatorDias, Jailson C
dc.creatorSuzuki, Erika
dc.creatorde Albuquerque, Cibele L
dc.creatorFerreira, Anderson L
dc.creatorBrito, Alba R M S
dc.creatorKubota, Lauro T
dc.date2009-May
dc.date2015-11-27T13:15:00Z
dc.date2015-11-27T13:15:00Z
dc.date.accessioned2018-03-29T01:08:29Z
dc.date.available2018-03-29T01:08:29Z
dc.identifierJournal Of Pharmaceutical And Biomedical Analysis. v. 49, n. 4, p. 1128-32, 2009-May.
dc.identifier1873-264X
dc.identifier10.1016/j.jpba.2009.02.013
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19297112
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198228
dc.identifier19297112
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298461
dc.descriptionA new chromatographic method for the sequential determination of short-chain fatty acids is described. Acetic, propionic and butyric acids were determined in dietary fiber extracts using ion-exclusion chromatography equipped with inverse chemical suppression and conductivity detection. The best optimization of the chromatographic conditions were achieved when a 100 mm x 7.8 mm ion-exclusion column with a solution of 0.5 mmol L(-1) sulfuric acid as eluent in a flow rate of 0.6 mL min(-1) were employed. The organic acids were sequentially separated in less than 10 min with limits of detection ranging from 1 up to 7.5 micromol L(-1) and limits of quantification from 5 up to 25 micromol L(-1). The linearity of the analytical response was studied in the range of 0.005-10 mmol L(-1) for acetic acid and 0.025-10 mmol L(-1) for propionic and butyric acids with coefficients of determination (R(2)) ranging between 0.9985 and 0.9999. The method was tested and proved to be selective, precise, accurate, reproducible and highly sensitive. Finally, the method was applied in the analysis of biological samples.
dc.description49
dc.description1128-32
dc.languageeng
dc.relationJournal Of Pharmaceutical And Biomedical Analysis
dc.relationJ Pharm Biomed Anal
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectCations
dc.subjectChromatography, Ion Exchange
dc.subjectDietary Fiber
dc.subjectElectric Conductivity
dc.subjectFatty Acids
dc.subjectFeces
dc.subjectFermentation
dc.subjectHumans
dc.subjectIndicators And Reagents
dc.subjectReproducibility Of Results
dc.titleDetermination Of Short-chain Fatty Acids In Dietary Fiber Extracts Using Ion-exclusion Chromatography With Suppressed Conductivity Detection.
dc.typeArtículos de revistas


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