Schistosoma Mansoni: Evaluation Of An Rnai-based Treatment Targeting Hgprtase Gene.

dc.creatorPereira, T C
dc.creatorPascoal, V D B
dc.creatorMarchesini, R B
dc.creatorMaia, I G
dc.creatorMagalhães, L A
dc.creatorZanotti-Magalhães, E M
dc.creatorLopes-Cendes, I
dc.date2008-Apr
dc.date2015-11-27T13:13:36Z
dc.date2015-11-27T13:13:36Z
dc.date.accessioned2018-03-29T01:08:16Z
dc.date.available2018-03-29T01:08:16Z
dc.identifierExperimental Parasitology. v. 118, n. 4, p. 619-23, 2008-Apr.
dc.identifier0014-4894
dc.identifier10.1016/j.exppara.2007.11.017
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/18237732
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198174
dc.identifier18237732
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298407
dc.descriptionHypoxanthine-guanine phosphoribosyltransferase (HGPRTase) is an essential gene of the parasite Schistosoma mansoni and it is well conserved in its hosts (mouse and human) at the protein but not at the RNA level. This feature prompted us to assess RNA interference (RNAi) to combat schistosomiasis. Small interfering RNAs (siRNAs) were produced against HGPRTase, injected in infected mice and the number of worms was counted six days after injection. The total number of parasites was reduced by approximately 27% after treatment. RT-PCR analyzes showed a significant reduction in parasite target mRNA but not in host's homologue. The use of low doses of molecules did not oversaturate si- or miRNA pathways as mice survival rates were not affected by siRNAs. This is the first successful in vivo demonstration of a RNAi-based treatment against schistosomiasis. We believe that improvements in molecule delivery and an increase on siRNA dose could rapidly eliminate parasite.
dc.description118
dc.description619-23
dc.languageeng
dc.relationExperimental Parasitology
dc.relationExp. Parasitol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectFemale
dc.subjectHumans
dc.subjectHypoxanthine Phosphoribosyltransferase
dc.subjectInjections, Intravenous
dc.subjectMice
dc.subjectRna Interference
dc.subjectRna, Small Interfering
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectSchistosoma Mansoni
dc.subjectSchistosomiasis Mansoni
dc.subjectSpecies Specificity
dc.titleSchistosoma Mansoni: Evaluation Of An Rnai-based Treatment Targeting Hgprtase Gene.
dc.typeArtículos de revistas


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