Relaxation Of The Mouse Pubic Symphysis During Late Pregnancy Is Not Accompanied By The Influx Of Granulocytes.

dc.creatorRosa, Renata Giardini
dc.creatorTarsitano, Christiane Aparecida Badin
dc.creatorHyslop, Stephen
dc.creatorYamada, Aureo Tatsumi
dc.creatorToledo, Olga Maria S
dc.creatorJoazeiro, Paulo Pinto
dc.date2008-Mar
dc.date2015-11-27T13:13:26Z
dc.date2015-11-27T13:13:26Z
dc.date.accessioned2018-03-29T01:07:57Z
dc.date.available2018-03-29T01:07:57Z
dc.identifierMicroscopy Research And Technique. v. 71, n. 3, p. 169-78, 2008-Mar.
dc.identifier1059-910X
dc.identifier10.1002/jemt.20549
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/18044701
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198093
dc.identifier18044701
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298326
dc.descriptionIn some animals, such as mice and guinea pigs, a hormonally controlled mechanism increases the flexibility of the pubic symphysis and enhances the cervical remodeling necessary for safe delivery. Cervical ripening during pregnancy is associated with a paradoxical influx of leukocytes. However, the changes in cell metabolism during relaxation of the mouse pubic symphysis for delivery have not been extensively studied. In this work, we used light microscopy and transmission and scanning electron microcopy, as well as immunohistochemistry and Western blotting for MMP-8, to investigate the involvement of granulocytes or resident stromal cells in the relaxation of the virgin pubic symphysis during late pregnancy (days 18 and 19, before delivery) in vivo and in explanted joints. MMP-8 was studied because this collagenase is a hallmark for cervical ripening associated with the influx of granulocytes during late pregnancy. Extensive dissolution and disorganization of the extracellular matrix was seen around fibroblastic-like cells in late pregnancy. In contrast to the cervix (positive control), morphological and immunohistochemical analyses revealed that there was no characteristic cellular inflammatory response in the interpubic tissue. Staining for MMP-8 was observed in chondroid and fibroblastic-like cells of virgin and relaxed interpubic ligament, respectively. However, no granulocytes were seen during the extensive remodeling of the pubic joint in late pregnancy. These results indicate that constitutive stromal cells may have an important role in tissue relaxation during remodeling of the pubic symphysis in pregnancy.
dc.description71
dc.description169-78
dc.languageeng
dc.relationMicroscopy Research And Technique
dc.relationMicrosc. Res. Tech.
dc.rightsfechado
dc.rights(c) 2007 Wiley-Liss, Inc.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBlotting, Western
dc.subjectFemale
dc.subjectGranulocytes
dc.subjectImmunohistochemistry
dc.subjectMatrix Metalloproteinase 8
dc.subjectMice
dc.subjectMicroscopy, Electron
dc.subjectPregnancy
dc.subjectPregnancy, Animal
dc.subjectPubic Symphysis
dc.titleRelaxation Of The Mouse Pubic Symphysis During Late Pregnancy Is Not Accompanied By The Influx Of Granulocytes.
dc.typeArtículos de revistas


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