Actin Cytoskeletal And Functional Studies Of The Proximal Convoluted Tubules After Preservation.

dc.creatorFigueiredo, J F
dc.creatorBertels, I M V
dc.creatorGontijo, J A R
dc.date2008-Dec
dc.date2015-11-27T13:13:24Z
dc.date2015-11-27T13:13:24Z
dc.date.accessioned2018-03-29T01:07:53Z
dc.date.available2018-03-29T01:07:53Z
dc.identifierTransplantation Proceedings. v. 40, n. 10, p. 3311-5, 2008-Dec.
dc.identifier0041-1345
dc.identifier10.1016/j.transproceed.2008.06.060
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19100379
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198077
dc.identifier19100379
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298310
dc.descriptionProximal tubule cells have specialized apical membranes with microvilli that provide an extensive surface area for unidirectional transport of solute from lumen to blood. The major structural solute component is F-actin, which interacts with transmembrane proteins, including ion transport molecules related to normal absorptive and secretory functions. Our study was to evaluate F-actin and fluid absorption (Jv) in proximal tubules after exposure to preservation solutions. In vitro microperfusion technique and immunohistochemistry analysis. 1. Absorptions were similar in 1- and 24-hour-preserved tubules, as well as in fresh tubules. The exception was tubules for 24 hours in Euro-Collins solution, which did not show absorption, suggesting that it was affected. 2. Fluorescence intensity of actin tubules preserved for 1 hour in both solutions showed similar values to each other and to the control group; tubules preserved for 24 hours in both solutions were similar to each other, although statistically different than the control group and those preserved for 1 hour in Belzer (UW) solution. There were differences among groups in the distribution of F-actin; Jv values were different for 24-hour preservation in each solution, whereas fluorescence intensity was similar in both 24-hour solutions. Thus, actin cytoskeleton was not responsible for it, because 24-hour preservation in UW showed Jv results comparable to the control group.
dc.description40
dc.description3311-5
dc.languageeng
dc.relationTransplantation Proceedings
dc.relationTransplant. Proc.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAbsorption
dc.subjectActin Cytoskeleton
dc.subjectActins
dc.subjectAdenosine
dc.subjectAllopurinol
dc.subjectAnimals
dc.subjectCytoskeleton
dc.subjectGlutathione
dc.subjectInsulin
dc.subjectKidney Tubules, Collecting
dc.subjectKidney Tubules, Proximal
dc.subjectMale
dc.subjectMicroscopy, Confocal
dc.subjectMicrovilli
dc.subjectModels, Animal
dc.subjectOrgan Preservation
dc.subjectOrgan Preservation Solutions
dc.subjectPerfusion
dc.subjectRabbits
dc.subjectRaffinose
dc.titleActin Cytoskeletal And Functional Studies Of The Proximal Convoluted Tubules After Preservation.
dc.typeArtículos de revistas


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