Effects Of Systemic Administration Of Ciliary Neurotrophic Factor On Bax And Bcl-2 Proteins In The Lumbar Spinal Cord Of Neonatal Rats After Sciatic Nerve Transection.

dc.creatorRezende, A C S
dc.creatorVieira, A S
dc.creatorRogério, F
dc.creatorRezende, L F
dc.creatorBoschero, A C
dc.creatorNegro, A
dc.creatorLangone, F
dc.date2008-Nov
dc.date2015-11-27T13:13:21Z
dc.date2015-11-27T13:13:21Z
dc.date.accessioned2018-03-29T01:07:48Z
dc.date.available2018-03-29T01:07:48Z
dc.identifierBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 41, n. 11, p. 1024-8, 2008-Nov.
dc.identifier1414-431X
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19039380
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198053
dc.identifier19039380
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298286
dc.descriptionCiliary neurotrophic factor (CNTF) is a cytokine that plays a neuroprotective role in relation to axotomized motoneurons. We determined the effect of daily subcutaneous doses of CNTF (1.2 microg/g for 5 days; N = 13) or PBS (N = 13) on the levels of mRNA for Bcl-2 and Bax, as well as the expression and inter-association of Bcl-2 and Bax proteins, and the survival of motoneurons in the spinal cord lumbar enlargement of 2-day-old Wistar rats after sciatic nerve transection. Five days after transection, the effects were evaluated on histological and molecular levels using Nissl staining, immunoprecipitation, Western blot analysis, and reverse transcriptase-polymerase chain reaction. The motoneuron survival ratio, defined as the ratio between the number of motoneurons counted on the lesioned side vs those on the unlesioned side, was calculated. This ratio was 0.77 +/- 0.02 for CNTF-treated rats vs 0.53 +/- 0.02 for the PBS-treated controls (P < 0.001). Treatment with CNTF modified the level of mRNA, with the expression of Bax RNA decreasing 18% (with a consequent decrease in the level of Bax protein), while the expression of Bcl-2 RNA was increased 87%, although the level of Bcl-2 protein was unchanged. The amount of Bcl-2/Bax heterodimer increased 91% over that found in the PBS-treated controls. These data show, for the first time, that the neuroprotective effect of CNTF on neonatal rat axotomized motoneurons is associated with a reduction in free Bax, due to the inhibition of Bax expression, as well as increased Bcl-2/Bax heterodimerization. Thus, the neuroprotective action of the CNTF on axotomized motoneurons can be related to the inhibition of this apoptotic pathway.
dc.description41
dc.description1024-8
dc.languageeng
dc.relationBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]
dc.relationBraz. J. Med. Biol. Res.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAnimals, Newborn
dc.subjectBlotting, Western
dc.subjectCiliary Neurotrophic Factor
dc.subjectImmunoprecipitation
dc.subjectProto-oncogene Proteins C-bcl-2
dc.subjectRna, Messenger
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectSciatic Nerve
dc.subjectSpinal Cord
dc.subjectBcl-2-associated X Protein
dc.titleEffects Of Systemic Administration Of Ciliary Neurotrophic Factor On Bax And Bcl-2 Proteins In The Lumbar Spinal Cord Of Neonatal Rats After Sciatic Nerve Transection.
dc.typeArtículos de revistas


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