Islet Neogenesis-associated Protein Signaling In Neonatal Pancreatic Rat Islets: Involvement Of The Cholinergic Pathway.

dc.creatorBarbosa, Helena C
dc.creatorBordin, Silvana
dc.creatorAnhê, Gabriel
dc.creatorPersaud, Shanta J
dc.creatorBowe, James
dc.creatorBorelli, Maria I
dc.creatorGagliardino, Juan J
dc.creatorBoschero, Antonio C
dc.date2008-Nov
dc.date2015-11-27T13:13:10Z
dc.date2015-11-27T13:13:10Z
dc.date.accessioned2018-03-29T01:07:28Z
dc.date.available2018-03-29T01:07:28Z
dc.identifierThe Journal Of Endocrinology. v. 199, n. 2, p. 299-306, 2008-Nov.
dc.identifier1479-6805
dc.identifier10.1677/JOE-08-0309
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/18772167
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197966
dc.identifier18772167
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298199
dc.descriptionIslet neogenesis associated protein (INGAP) increases islet mass and insulin secretion in neonatal and adult rat islets. In the present study, we measured the short- and long-term effects of INGAP-PP (a pentadecapeptide having the 104-118 amino acid sequence of INGAP) upon islet protein expression and phosphorylation of components of the PI3K, MAPK and cholinergic pathways, and on insulin secretion. Short-term exposure of neonatal islets to INGAP-PP (90 s, 5, 15, and 30 min) significantly increased Akt1(-Ser473) and MAPK3/1(-Thr202/Tyr204) phosphorylation and INGAP-PP also acutely increased insulin secretion from islets perifused with 2 and 20 mM glucose. Islets cultured for 4 days in the presence of INGAP-PP showed an increased expression of Akt1, Frap1, and Mapk1 mRNAs as well as of the muscarinic M3 receptor subtype, and phospholipase C (PLC)-beta2 proteins. These islets also showed increased Akt1 and MAPK3/1 protein phosphorylation. Brief exposure of INGAP-PP-treated islets to carbachol (Cch) significantly increased P70S6K(-Thr389) and MAPK3/1 phosphorylation and these islets released more insulin when challenged with Cch that was prevented by the M3 receptor antagonist 4-DAMP, in a concentration-dependent manner. In conclusion, these data indicate that short- and long-term exposure to INGAP-PP significantly affects the expression and the phosphorylation of proteins involved in islet PI3K and MAPK signaling pathways. The observations of INGAPP-PP-stimulated up-regulation of cholinergic M3 receptors and PLC-beta2 proteins, enhanced P70S6K and MAPK3/1 phosphorylation and Cch-induced insulin secretion suggest a participation of the cholinergic pathway in INGAP-PP-mediated effects.
dc.description199
dc.description299-306
dc.languageeng
dc.relationThe Journal Of Endocrinology
dc.relationJ. Endocrinol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAnimals, Newborn
dc.subjectAntigens, Neoplasm
dc.subjectCarbachol
dc.subjectCarrier Proteins
dc.subjectImmunoblotting
dc.subjectIn Vitro Techniques
dc.subjectInsulin
dc.subjectIslets Of Langerhans
dc.subjectLectins, C-type
dc.subjectMitogen-activated Protein Kinase 1
dc.subjectMitogen-activated Protein Kinase 3
dc.subjectPhosphorylation
dc.subjectPhosphotransferases (alcohol Group Acceptor)
dc.subjectPiperidines
dc.subjectProto-oncogene Proteins C-akt
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRibosomal Protein S6 Kinases, 70-kda
dc.subjectSignal Transduction
dc.subjectTor Serine-threonine Kinases
dc.subjectTumor Markers, Biological
dc.titleIslet Neogenesis-associated Protein Signaling In Neonatal Pancreatic Rat Islets: Involvement Of The Cholinergic Pathway.
dc.typeArtículos de revistas


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