Assessment Of The Diagnostic Potential Of Immmunocapture-pcr And Immuno-pcr For Citrus Variegated Chlorosis.

dc.creatorPeroni, Luís Antonio
dc.creatorReis, José Raimundo Ribeiro Dos
dc.creatorColetta-Filho, Helvécio Della
dc.creatorde Souza, Alessandra Alves
dc.creatorMachado, Marcos Antonio
dc.creatorStach-Machado, Dagmar Ruth
dc.date2008-Oct
dc.date2015-11-27T13:13:03Z
dc.date2015-11-27T13:13:03Z
dc.date.accessioned2018-03-29T01:07:14Z
dc.date.available2018-03-29T01:07:14Z
dc.identifierJournal Of Microbiological Methods. v. 75, n. 2, p. 302-7, 2008-Oct.
dc.identifier0167-7012
dc.identifier10.1016/j.mimet.2008.06.024
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/18656503
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197910
dc.identifier18656503
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298143
dc.descriptionXylella fastidiosa causes significant losses in many economically important crops. An efficient pathogen detection system is critical for epidemiology studies, particularly when large sample size is involved. In this study we report the development of immunomolecular assays like Immmunocapture-PCR and Immuno-PCR for direct detection of X. fastidiosa without DNA isolation. Whereas the reactivity of ELISA and PCR ranged from 10(6) to 10(4) bacterial cells, the IC-PCR sensitivity was up to 10(3) and the detection limit of I-PCR was up to 10(1) bacterial cells. These methods can use either plant sample extracts or cultivated media, and show no cross reaction for any other endophytic citrus-bacteria. Therefore, IC-PCR and I-PCR assays provide an alternative for quick and very sensitive methods to screening X. fastidiosa, with the advantage of not requiring any concentration or DNA purification steps while still allowing an accurate diagnosis of CVC.
dc.description75
dc.description302-7
dc.languageeng
dc.relationJournal Of Microbiological Methods
dc.relationJ. Microbiol. Methods
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAntibodies, Bacterial
dc.subjectCitrus Sinensis
dc.subjectCulture Media
dc.subjectEnzyme-linked Immunosorbent Assay
dc.subjectPlant Diseases
dc.subjectPlant Leaves
dc.subjectPolymerase Chain Reaction
dc.subjectSensitivity And Specificity
dc.subjectXylella
dc.titleAssessment Of The Diagnostic Potential Of Immmunocapture-pcr And Immuno-pcr For Citrus Variegated Chlorosis.
dc.typeArtículos de revistas


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