Optimization Of R-(+)-alpha-terpineol Production By The Biotransformation Of R-(+)-limonene.

dc.creatorBicas, Juliano Lemos
dc.creatorBarros, Francisco Fábio Cavalcante
dc.creatorWagner, Roger
dc.creatorGodoy, Helena Teixeira
dc.creatorPastore, Gláucia Maria
dc.date2008-Sep
dc.date2015-11-27T13:12:55Z
dc.date2015-11-27T13:12:55Z
dc.date.accessioned2018-03-29T01:07:00Z
dc.date.available2018-03-29T01:07:00Z
dc.identifierJournal Of Industrial Microbiology & Biotechnology. v. 35, n. 9, p. 1061-70, 2008-Sep.
dc.identifier1367-5435
dc.identifier10.1007/s10295-008-0383-0
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/18560915
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197847
dc.identifier18560915
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298080
dc.descriptionR-(+)-limonene is an abundant and non-expensive by-product of the citrus industry and is, therefore, a suitable starting material for the production of natural flavor and fragrance compounds. The biotransformation of R-(+)-limonene to R-(+)-alpha-terpineol by Fusarium oxysporum 152b has already been reported, although the influence of the main process parameters on the production has not yet been evaluated. In this paper, a Plackett-Burman screening design was used to define the effects of the medium composition (glucose, peptone, yeast extract, malt extract and pH), the presence of a co-substrate (biosurfactant), the cultivation conditions (temperature, agitation), the substrate concentration and the inoculum/culture medium ratio on the absolute amount of R-(+)-alpha-terpineol resulting from this biotransformation. The process conditions were further optimized applying response surface methodology (RSM). The volatiles were extracted using a SPME device and were subsequently quantified by GC-FID and identified by GC-MS. The best results were obtained using 0.5% (v/m) R-(+)-limonene in pure distilled water as the culture medium with an inoculum/culture medium ratio of 0.25 (m/m) and 72 h cultivation at 26 degrees C/240 rpm. Under these conditions the concentration of R-(+)-alpha-terpineol in the culture medium reached 2.4 g L(-1), a production almost six times greater than in earlier trials. The presence of a biosurfactant (0-500 mg L(-1)) did not significantly increase the yield.
dc.description35
dc.description1061-70
dc.languageeng
dc.relationJournal Of Industrial Microbiology & Biotechnology
dc.relationJ. Ind. Microbiol. Biotechnol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnalysis Of Variance
dc.subjectBiotransformation
dc.subjectCulture Media
dc.subjectCyclohexenes
dc.subjectFood Technology
dc.subjectFusarium
dc.subjectLeast-squares Analysis
dc.subjectMonoterpenes
dc.subjectTemperature
dc.subjectTerpenes
dc.titleOptimization Of R-(+)-alpha-terpineol Production By The Biotransformation Of R-(+)-limonene.
dc.typeArtículos de revistas


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