Increased Adhesive Properties Of Neutrophils In Sickle Cell Disease May Be Reversed By Pharmacological Nitric Oxide Donation.

dc.creatorCanalli, Andreia A
dc.creatorFranco-Penteado, Carla F
dc.creatorSaad, Sara T O
dc.creatorConran, Nicola
dc.creatorCosta, Fernando F
dc.date2008-Apr
dc.date2015-11-27T13:12:33Z
dc.date2015-11-27T13:12:33Z
dc.date.accessioned2018-03-29T01:06:23Z
dc.date.available2018-03-29T01:06:23Z
dc.identifierHaematologica. v. 93, n. 4, p. 605-9, 2008-Apr.
dc.identifier1592-8721
dc.identifier10.3324/haematol.12119
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/18326523
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197696
dc.identifier18326523
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297929
dc.descriptionIncreased leukocyte adhesion to vascular endothelium contributes to vaso-occlusion in sickle cell disease. Since nitric oxide bioavailability is decreased in sickle cell disease and nitric oxide may inhibit leukocyte adhesion, we investigated whether stimulation of NO-signaling pathways can reduce the adhesive properties of neutrophils from sickle cell disease individuals (sickle cell diseaseneu). sickle cell diseaseneu presented greater adhesion in vitro to both fibronectin and ICAM-1 than control neutrophils. Co-incubation of sickle cell diseaseneu with the nitric oxide-donor agents, sodium nitroprusside and dietheylamine NONOate (DEANO), and the guanylate cyclase stimulator, BAY41-2272, all significantly reduced the increased adhesion to fibronectin/ICAM-1. Oxadiazolo[4,3-a]quinoxalin-1-one, a guanylate cyclase inhibitor, reversed sodium nitroprusside/DEANO-diminished adhesion to fibronectin, implicating cGMP-dependent signaling in this mechanism. Interestingly, intracellular cGMP was significantly higher in neutrophils from sickle cell disease individuals on hydroxyurea (sickle cell diseaseHUneu). Accordingly, sickle cell diseaseHUneu adhesion to fibronectin/ICAM-1 was significantly lower than that of sickle cell diseaseneu. Agents that stimulate the nitric oxide/cGMP-dependent pathway may have beneficial effects on leukocyte function if used in these subjects.
dc.description93
dc.description605-9
dc.languageeng
dc.relationHaematologica
dc.relationHaematologica
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAdult
dc.subjectAnemia, Sickle Cell
dc.subjectAntigens, Cd11a
dc.subjectAntigens, Cd11b
dc.subjectCell Adhesion
dc.subjectCyclic Gmp
dc.subjectEndothelial Cells
dc.subjectFemale
dc.subjectFibronectins
dc.subjectHumans
dc.subjectHydrazines
dc.subjectHydroxyurea
dc.subjectIntegrin Alpha4
dc.subjectIntercellular Adhesion Molecule-1
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectNeutrophils
dc.subjectNitric Oxide
dc.subjectNitric Oxide Donors
dc.subjectNitroprusside
dc.subjectOxadiazoles
dc.subjectPyrazoles
dc.subjectPyridines
dc.subjectQuinoxalines
dc.subjectSickle Cell Trait
dc.subjectAlpha-thalassemia
dc.titleIncreased Adhesive Properties Of Neutrophils In Sickle Cell Disease May Be Reversed By Pharmacological Nitric Oxide Donation.
dc.typeArtículos de revistas


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