Inhibition Of Avian Metapneumovirus (ampv) Replication By Rna Interference Targeting Nucleoprotein Gene (n) In Cultured Cells.

dc.creatorFerreira, Helena Lage
dc.creatorSpilki, Fernando Rosado
dc.creatorde Almeida, Renata Servan
dc.creatorSantos, Márcia M A B
dc.creatorArns, Clarice Weis
dc.date2007-Apr
dc.date2015-11-27T13:10:52Z
dc.date2015-11-27T13:10:52Z
dc.date.accessioned2018-03-29T01:06:13Z
dc.date.available2018-03-29T01:06:13Z
dc.identifierAntiviral Research. v. 74, n. 1, p. 77-81, 2007-Apr.
dc.identifier0166-3542
dc.identifier10.1016/j.antiviral.2006.12.002
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/17275932
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197653
dc.identifier17275932
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297886
dc.descriptionAvian metapneumovirus (AMPV) is the primary causative agent of severe rhinotracheitis in turkeys. It is associated with swollen head syndrome in chickens and is the source of significant economic losses to animal food production. In this study, we designed specific short interfering RNA (siRNA) targeting the AMPV nucleoprotein (N) and fusion (F) genes. Three days post-virus infection, virus titration, real time RT-PCR, and RT-PCR assays were performed to verify the effect of siRNA in AMPV replication. A marked decrease in virus titers from transfected CER cells treated with siRNA/N was observed. Also, the production of N, F, and G mRNAs in AMPV was decreased. Results indicate that N-specific siRNA can inhibit virus replication. In future studies, a combination of siRNAs targeting the RNA polymerase complex may be used as a tool to study AMPV replication and/or antiviral therapy.
dc.description74
dc.description77-81
dc.languageeng
dc.relationAntiviral Research
dc.relationAntiviral Res.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCell Line
dc.subjectChick Embryo
dc.subjectMetapneumovirus
dc.subjectParamyxoviridae Infections
dc.subjectRna Interference
dc.subjectRna, Small Interfering
dc.subjectRna, Viral
dc.subjectSpecies Specificity
dc.subjectTransfection
dc.subjectViral Fusion Proteins
dc.subjectViral Proteins
dc.subjectVirus Replication
dc.titleInhibition Of Avian Metapneumovirus (ampv) Replication By Rna Interference Targeting Nucleoprotein Gene (n) In Cultured Cells.
dc.typeArtículos de revistas


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