Dna Content And Chromatin Texture Of Human Breast Epithelial Cells Transformed With 17-beta-estradiol And The Estrogen Antagonist Ici 182,780 As Assessed By Image Analysis.

dc.creatorMello, Maria Luiza S
dc.creatorVidal, Benedicto C
dc.creatorRusso, Irma H
dc.creatorLareef, Mohamed H
dc.creatorRusso, Jose
dc.date2007-Apr
dc.date2015-11-27T13:10:51Z
dc.date2015-11-27T13:10:51Z
dc.date.accessioned2018-03-29T01:06:12Z
dc.date.available2018-03-29T01:06:12Z
dc.identifierMutation Research. v. 617, n. 1-2, p. 1-7, 2007-Apr.
dc.identifier0027-5107
dc.identifier10.1016/j.mrfmmm.2006.08.013
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/17270221
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197649
dc.identifier17270221
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297882
dc.descriptionThe immortalized human breast epithelial MCF-10F cell line, although estrogen receptor alpha negative, develops cell proliferating activities and invasiveness indicative of neoplastic transformation, after treatment with 17-beta-estradiol (E-2). These effects are similar to those produced by benzo[a]pyrene (BP). Since we have previously reported changes in the nuclear parameters accompanying BP-induced tumorigenesis in MCF-10F cells, we have examined whether similar alterations occur in E-2-treated cells. We therefore studied DNA amounts and other nuclear parameters in Feulgen-stained MCF-10F cells after treatment with various concentrations of E-2, BP, the estrogen antagonist ICI 182,780, and E-2 in the presence of ICI 182,780. E-2 caused a certain loss of DNA and changes in the nuclear size and chromatin supraorganization of MCF-10F cells. Many of these changes were similar to those produced by BP and were indicative of neoplastic transformation. More intense chromatin remodelling was seen with 70 nM E-2. Since these changes were not abrogated totally or partially by ICI 182,780, the neoplastic transformation of MCF-10F cells stimulated by E-2 involved a process that was independent of estrogen alpha-receptors. The changes produced by ICI 182,780 alone were attributed to effects other than its well-known anti-estrogenic activity.
dc.description617
dc.description1-7
dc.languageeng
dc.relationMutation Research
dc.relationMutat. Res.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectBenzo(a)pyrene
dc.subjectBreast
dc.subjectBreast Neoplasms
dc.subjectCell Line, Transformed
dc.subjectCell Nucleus
dc.subjectCell Transformation, Neoplastic
dc.subjectChromatin
dc.subjectDna, Neoplasm
dc.subjectEpithelial Cells
dc.subjectEstradiol
dc.subjectEstrogen Antagonists
dc.subjectFemale
dc.subjectHumans
dc.subjectImage Cytometry
dc.titleDna Content And Chromatin Texture Of Human Breast Epithelial Cells Transformed With 17-beta-estradiol And The Estrogen Antagonist Ici 182,780 As Assessed By Image Analysis.
dc.typeArtículos de revistas


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