| dc.creator | Santos, Lucielen Oliveira | |
| dc.creator | Gonzales, Tatiane Araujo | |
| dc.creator | Ubeda, Beatriz Torsani | |
| dc.creator | Monte Alegre, Ranulfo | |
| dc.date | 2007-Dec | |
| dc.date | 2015-11-27T13:10:33Z | |
| dc.date | 2015-11-27T13:10:33Z | |
| dc.date.accessioned | 2018-03-29T01:05:37Z | |
| dc.date.available | 2018-03-29T01:05:37Z | |
| dc.identifier | Applied Microbiology And Biotechnology. v. 77, n. 4, p. 763-9, 2007-Dec. | |
| dc.identifier | 0175-7598 | |
| dc.identifier | 10.1007/s00253-007-1211-6 | |
| dc.identifier | http://www.ncbi.nlm.nih.gov/pubmed/17926030 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/197496 | |
| dc.identifier | 17926030 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1297729 | |
| dc.description | A strategy of experimental design using a fractional factorial design (FFD) and a central composite rotatable design (CCRD) were carried out with the aim to obtain the best conditions of temperature (20-30 degrees C), agitation rate (100-300 rpm), initial pH (5.0-7.0), inoculum concentration (5-15%), and glucose concentration (30-70 g/l) for glutathione (GSH) production in shake-flask culture by Saccharomyces cerevisiae ATCC 7754. By a FFD (2(5-2)), the agitation rate, temperature, and pH were found to be significant factors for GSH production. In CCRD (2(2)) was obtained a second-order model equation, and the percent of variation explained by the model was 95%. The results showed that the optimal culture conditions were agitation rate, 300 rpm; temperature, 20 degrees C; initial pH, 5; glucose, 54 g/l; and inoculum concentration, 5%. The highest GSH concentration (154.5 mg/l) was obtained after 72 h of fermentation. | |
| dc.description | 77 | |
| dc.description | 763-9 | |
| dc.language | eng | |
| dc.relation | Applied Microbiology And Biotechnology | |
| dc.relation | Appl. Microbiol. Biotechnol. | |
| dc.rights | fechado | |
| dc.rights | | |
| dc.source | PubMed | |
| dc.subject | Biotechnology | |
| dc.subject | Culture Media | |
| dc.subject | Fermentation | |
| dc.subject | Glucose | |
| dc.subject | Glutathione | |
| dc.subject | Hydrogen-ion Concentration | |
| dc.subject | Saccharomyces Cerevisiae | |
| dc.subject | Temperature | |
| dc.title | Influence Of Culture Conditions On Glutathione Production By Saccharomyces Cerevisiae. | |
| dc.type | Artículos de revistas | |
