Artículos de revistas
Simultaneous Quantification Of Gmp, Amp, Cyclic Gmp And Cyclic Amp By Liquid Chromatography Coupled To Tandem Mass Spectrometry.
Registro en:
Journal Of Chromatography. B, Analytical Technologies In The Biomedical And Life Sciences. v. 859, n. 1, p. 37-41, 2007-Nov.
1570-0232
10.1016/j.jchromb.2007.09.008
17919998
Autor
Lorenzetti, Raquel
Lilla, Sergio
Donato, José Luis
de Nucci, Gilberto
Institución
Resumen
Phosphodiesterases are drug targets for treating various diseases. Inhibition of these can increase cAMP and cGMP levels, which can affect a variety of physiological responses. Here we report a new method for determining PDE activity by combining high-performance liquid chromatography and tandem mass spectrometry. Characteristic peaks of the substrates, cGMP or cAMP and products, GMP or AMP, were identified in positive-ion electrospray ionization using multiple reaction monitoring. The method can be applied to determine activity of PDE inhibitors. Our results showed that this new method was fast, sensitive and highly reproducible. 859 37-41