Induction Of Apoptosis In Caco-2 And Ht-29 Human Intestinal Epithelial Cells By Enterohemolysin Produced By Classic Enteropathogenic Escherichia Coli.

dc.creatorFigueiredo, P M S
dc.creatorFurumura, M T
dc.creatorAidar-Ugrinovich, L
dc.creatorPestana de Castro, A F
dc.creatorPereira, F G
dc.creatorMetze, I L
dc.creatorYano, T
dc.date2007-Oct
dc.date2015-11-27T13:10:27Z
dc.date2015-11-27T13:10:27Z
dc.date.accessioned2018-03-29T01:05:27Z
dc.date.available2018-03-29T01:05:27Z
dc.identifierLetters In Applied Microbiology. v. 45, n. 4, p. 358-63, 2007-Oct.
dc.identifier0266-8254
dc.identifier10.1111/j.1472-765X.2007.02154.x
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/17868319
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197451
dc.identifier17868319
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297684
dc.descriptionDetect the cytotoxic effects of the Enterohemolysin from enteropathogenic Escherichia coli C3888 (O 26: H-) on Caco 2 and HT-29-human epithelial intestinal cells. The Caco 2 and HT-29 cells, which were treated with Enterohemolysin (EHly) within 10-15 min, became round, lost attachment to substrate, showed extensive surface blebbing, nucleus shrank, and the chromatin became more compact. After 10 min of exposure to the EHly, the cells showed lactate dehydrogenase (LDH) leakage and reduction of mitochondrial activity. The cells showed disorganization of the actin fibers at 15 min. The death of these human epithelial intestinal cells by apoptosis was confirmed by annexin V. Enterohemolysin induced apoptosis on human epithelial intestinal cells. The finding of EHly cytotoxic activity suggests the involvement of this hemolysin in the (Enteropathogenic Escherichia coli) EPEC infection mechanism and may facilitate the understanding of the diarrhea caused by EPEC.
dc.description45
dc.description358-63
dc.languageeng
dc.relationLetters In Applied Microbiology
dc.relationLett. Appl. Microbiol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAlkaline Phosphatase
dc.subjectApoptosis
dc.subjectCaco-2 Cells
dc.subjectCell Survival
dc.subjectDiarrhea
dc.subjectEscherichia Coli
dc.subjectEscherichia Coli Infections
dc.subjectEscherichia Coli Proteins
dc.subjectHt29 Cells
dc.subjectHemolysin Proteins
dc.subjectHumans
dc.subjectIntestinal Mucosa
dc.subjectL-lactate Dehydrogenase
dc.titleInduction Of Apoptosis In Caco-2 And Ht-29 Human Intestinal Epithelial Cells By Enterohemolysin Produced By Classic Enteropathogenic Escherichia Coli.
dc.typeArtículos de revistas


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