Analysis Of Adenosine By Rp-hplc Method And Its Application To The Study Of Adenosine Kinase Kinetics.

dc.creatorMarin, Rodrigo Miguel
dc.creatorFranchini, Kleber Gomes
dc.creatorRocco, Silvana Aparecida
dc.date2007-Oct
dc.date2015-11-27T13:10:25Z
dc.date2015-11-27T13:10:25Z
dc.date.accessioned2018-03-29T01:05:24Z
dc.date.available2018-03-29T01:05:24Z
dc.identifierJournal Of Separation Science. v. 30, n. 15, p. 2473-9, 2007-Oct.
dc.identifier1615-9306
dc.identifier10.1002/jssc.200700194
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/17763527
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197439
dc.identifier17763527
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297672
dc.descriptionAn RP-HPLC method for the analysis of adenosine (ADO) has been developed and validated. In the present study, we report an RP-HPLC-based method with modifications of mobile phase and shorter retention time that substantially improved the efficiency of ADO analysis. The HPLC separation of the ADO was achieved on a C18 column, using a mobile phase consisting of water, containing 7% v/v ACN, at a flow rate of 0.8 mL/min. The column effluent was monitored by UV detection at 260 nm. A linear response was achieved over the concentration range of 0.25-100.00 micromol/L. The analytical method inter- and intra-run accuracy and precision were better than +/- 15%. The LOQ was 0.25 micromol/L, with ADO detection in the range of 6.25 pmol per sample. The method has been applied to the study of adenosine kinase (AK) kinetics.
dc.description30
dc.description2473-9
dc.languageeng
dc.relationJournal Of Separation Science
dc.relationJ Sep Sci
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAdenosine
dc.subjectAdenosine Kinase
dc.subjectAnimals
dc.subjectChemistry Techniques, Analytical
dc.subjectChromatography
dc.subjectChromatography, High Pressure Liquid
dc.subjectKinetics
dc.subjectMice
dc.subjectQuality Control
dc.subjectReproducibility Of Results
dc.subjectSensitivity And Specificity
dc.subjectSpectrophotometry, Ultraviolet
dc.subjectTime Factors
dc.subjectWater
dc.titleAnalysis Of Adenosine By Rp-hplc Method And Its Application To The Study Of Adenosine Kinase Kinetics.
dc.typeArtículos de revistas


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