Dexamethasone-induced Insulin Resistance Is Associated With Increased Connexin 36 Mrna And Protein Expression In Pancreatic Rat Islets.

dc.creatorRafacho, A
dc.creatorRoma, L P
dc.creatorTaboga, S R
dc.creatorBoschero, A C
dc.creatorBosqueiro, J R
dc.date2007-May
dc.date2015-11-27T13:10:18Z
dc.date2015-11-27T13:10:18Z
dc.date.accessioned2018-03-29T01:05:10Z
dc.date.available2018-03-29T01:05:10Z
dc.identifierCanadian Journal Of Physiology And Pharmacology. v. 85, n. 5, p. 536-45, 2007-May.
dc.identifier0008-4212
dc.identifier10.1139/y07-037
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/17632589
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197381
dc.identifier17632589
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297614
dc.descriptionAugmented glucose-stimulated insulin secretion (GSIS) is an adaptive mechanism exhibited by pancreatic islets from insulin-resistant animal models. Gap junction proteins have been proposed to contribute to islet function. As such, we investigated the expression of connexin 36 (Cx36), connexin 43 (Cx43), and the glucose transporter Glut2 at mRNA and protein levels in pancreatic islets of dexamethasone (DEX)-induced insulin-resistant rats. Study rats received daily injections of DEX (1 mg/kg body mass, i.p.) for 5 days, whereas control rats (CTL) received saline solution. DEX rats exhibited peripheral insulin resistance, as indicated by the significant postabsorptive insulin levels and by the constant rate for glucose disappearance (KITT). GSIS was significantly higher in DEX islets (1.8-fold in 16.7 mmol/L glucose vs. CTL, p < 0.05). A significant increase of 2.25-fold in islet area was observed in DEX vs. CTL islets (p < 0.05). Cx36 mRNA expression was significantly augmented, Cx43 diminished, and Glut2 mRNA was unaltered in islets of DEX vs. CTL (p < 0.05). Cx36 protein expression was 1.6-fold higher than that of CTL islets (p < 0.05). Glut2 protein expression was unaltered and Cx43 was not detected at the protein level. We conclude that DEX-induced insulin resistance is accompanied by increased GSIS and this may be associated with increase of Cx36 protein expression.
dc.description85
dc.description536-45
dc.languageeng
dc.relationCanadian Journal Of Physiology And Pharmacology
dc.relationCan. J. Physiol. Pharmacol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectArea Under Curve
dc.subjectBlood Glucose
dc.subjectConnexin 43
dc.subjectConnexins
dc.subjectDexamethasone
dc.subjectGene Expression
dc.subjectGlucocorticoids
dc.subjectGlucose
dc.subjectGlucose Tolerance Test
dc.subjectImmunoblotting
dc.subjectImmunohistochemistry
dc.subjectInjections, Intraperitoneal
dc.subjectInsulin
dc.subjectInsulin Resistance
dc.subjectIslets Of Langerhans
dc.subjectMale
dc.subjectRna, Messenger
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.titleDexamethasone-induced Insulin Resistance Is Associated With Increased Connexin 36 Mrna And Protein Expression In Pancreatic Rat Islets.
dc.typeArtículos de revistas


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