High Bcl-2/bax Ratio In Walker Tumor Cells Protects Mitochondria But Does Not Prevent H2o2-induced Apoptosis Via Calcineurin Pathways.

dc.creatorZecchin, Karina G
dc.creatorSeidinger, Ana Luiza O
dc.creatorChiaratti, Marcos R
dc.creatorDegasperi, Giovanna R
dc.creatorMeirelles, Flávio V
dc.creatorCastilho, Roger F
dc.creatorVercesi, Aníbal E
dc.date2007-Apr
dc.date2015-11-27T13:10:00Z
dc.date2015-11-27T13:10:00Z
dc.date.accessioned2018-03-29T01:04:39Z
dc.date.available2018-03-29T01:04:39Z
dc.identifierJournal Of Bioenergetics And Biomembranes. v. 39, n. 2, p. 186-94, 2007-Apr.
dc.identifier0145-479X
dc.identifier10.1007/s10863-007-9076-z
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/17431754
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197247
dc.identifier17431754
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297480
dc.descriptionIt has been previously shown that Walker 256 tumor cells express a high content of the anti-apoptotic protein Bcl-2 which protects mitochondria against the damaging effects of Ca(2+). In the present study, we analyze H(2)O(2)-induced apoptotic death in two different types of tumor cells: Walker 256 and SCC-25. Treatment with H(2)O(2) (4mM) increased reactive oxygen species generation and the concentration of cytosolic free Ca(2+). These alterations preceded apoptosis in both cell lines. In Walker cells, which show a high Bcl-2/Bax ratio, apoptosis was dependent on calcineurin activation and independent of changes in mitochondrial membrane potential (DeltaPsi(m)), as well as cytochrome c release. In contrast, in SCC-25 cells, which show a lower Bcl-2/Bax ratio, apoptosis was preceded by a decrease in DeltaPsi(m), mitochondrial permeability transition, and cytochrome c release. Caspase-3 activation occurred in both cell lines. The data suggest that although the high Bcl-2/Bax ratio protected the mitochondria of Walker cells from oxidative stress, it was not sufficient to prevent apoptosis through calcineurin pathways.
dc.description39
dc.description186-94
dc.languageeng
dc.relationJournal Of Bioenergetics And Biomembranes
dc.relationJ. Bioenerg. Biomembr.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectCalcineurin
dc.subjectCarcinoma 256, Walker
dc.subjectCaspase 3
dc.subjectCell Line, Tumor
dc.subjectCytochromes C
dc.subjectHumans
dc.subjectHydrogen Peroxide
dc.subjectMale
dc.subjectMembrane Potential, Mitochondrial
dc.subjectMitochondria
dc.subjectOxidative Stress
dc.subjectProto-oncogene Proteins C-bcl-2
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReactive Oxygen Species
dc.subjectSignal Transduction
dc.subjectBcl-2-associated X Protein
dc.titleHigh Bcl-2/bax Ratio In Walker Tumor Cells Protects Mitochondria But Does Not Prevent H2o2-induced Apoptosis Via Calcineurin Pathways.
dc.typeArtículos de revistas


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