Fluorescence, Birefringence And Confocal Microscopy Of The Abdominal Aorta From Nonobese Diabetic (nod) Mice.

dc.creatorAldrovani, Marcela
dc.creatorAparecida Guaraldo, Ana Maria
dc.creatorde Campos Vidal, Benedicto
dc.date2007
dc.date2015-11-27T13:09:53Z
dc.date2015-11-27T13:09:53Z
dc.date.accessioned2018-03-29T01:04:29Z
dc.date.available2018-03-29T01:04:29Z
dc.identifierActa Histochemica. v. 109, n. 3, p. 248-54, 2007.
dc.identifier0065-1281
dc.identifier10.1016/j.acthis.2007.01.003
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/17360029
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197204
dc.identifier17360029
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297437
dc.descriptionIn this study, nonenzymatic glycosylation was assessed in aorta extracellular matrix (ECM) from nonobese diabetic (NOD) mice, using nitroblue tetrazolium (NBT). Molecular and structural changes were investigated in elastic lamellae and collagen fibers of diabetic mice aortas after staining with dansyl chloride and anilinonaphthalene sulfonate (ANS). Alterations in arterial autofluorescence and birefringence of collagen fibers were investigated in unstained aortas. Proliferation of smooth muscle cells (SMC) was also investigated by Feulgen reaction staining assessed by confocal microscopy and image analysis. Assessment of nonenzymatic glycosylation demonstrated glycosylation products in the aorta ECM of NOD mice. Elastic lamellae and collagen fibers from NOD mouse aortas presented less intense fluorescence after staining with dansyl chloride and ANS when compared to aortas of control nondiabetic mice. However, unstained NOD aortas showed more intense autofluorescence when compared to controls. Birefringence analysis suggests alterations in the higher molecular packing of the arterial collagen fibers in NOD aortas. In aortas stained by Feulgen reaction, no evidence of SMC proliferation was observed in diabetic aortas.
dc.description109
dc.description248-54
dc.languageeng
dc.relationActa Histochemica
dc.relationActa Histochem.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAorta, Abdominal
dc.subjectBirefringence
dc.subjectCell Proliferation
dc.subjectCollagen
dc.subjectDansyl Compounds
dc.subjectDiabetes Mellitus, Type 1
dc.subjectElastic Tissue
dc.subjectGlycosylation
dc.subjectIndicators And Reagents
dc.subjectMice
dc.subjectMice, Inbred Nod
dc.subjectMicroscopy, Confocal
dc.subjectMicroscopy, Fluorescence
dc.subjectMuscle, Smooth, Vascular
dc.subjectNitroblue Tetrazolium
dc.subjectStaining And Labeling
dc.titleFluorescence, Birefringence And Confocal Microscopy Of The Abdominal Aorta From Nonobese Diabetic (nod) Mice.
dc.typeArtículos de revistas


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