[the Effect Of Bacterial Lipopolysaccharide On The Gastric Emptying Of Rats: A Pretreatment Evaluation Using Nw-nitro-l-arginine Methyl Ester (l-name)].

dc.creatorCollares, Edgard Ferro
dc.creatorVinagre, Adriana Mendes
dc.date
dc.date2015-11-27T13:06:05Z
dc.date2015-11-27T13:06:05Z
dc.date.accessioned2018-03-29T01:03:52Z
dc.date.available2018-03-29T01:03:52Z
dc.identifierArquivos De Gastroenterologia. v. 43, n. 3, p. 229-32
dc.identifier0004-2803
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/17160240
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197038
dc.identifier17160240
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297271
dc.descriptionThere is evidence that nitric oxide plays a role in the decrease in gastric emptying induced by bacterial lipopolysaccharide. To evaluate the effect of pretreatment with Nw-nitro-L-arginine methyl to ester, one competitive inhibitor of the nitric oxide synthases, on the gastric emptying delay induced by lipopolysaccharide. Male Wistar rats, SPF, were used after 24 h fast and 1 h-water withdrawn. The pretreatment was done intravenously with vehicle (saline) or N(w)-nitro-L-arginine methyl to ester in the doses of 0.5, 1, 2.5 e 5 mg/kg. After 10 min, the animals were treated iv with lipopolysaccharide (50 microg/kg) or received vehicle (saline). The gastric emptying was evaluated 1 h after the lipopolysaccharide administration. A saline solution containing phenol red was used as the test meal. The gastric emptying was indirectly assessed by the determination of percent gastric retention of the test meal 10 min after orogastric administration. The animals pretreated with vehicle and treatment with lipopolysaccharide have significant rise of the gastric retention (average = 57%) in comparison with the controls receiving only vehicle (38.1%). The pretreatment with the different doses of N(w)-nitro-L-arginine methyl to ester did not modify per se the gastric retention in comparison with the animals pretreated with vehicle. Pretreatment with N(w)-nitro-L-arginine methyl to ester with the dose of 1 mg/kg determined a discrete but significant reduction in the gastric retention (52%) of animals treated with lipopolysaccharide in comparison with vehicle-pretreated rats. Paradoxically, animals pretreated with 2.5 or 5 mg of N(w)-nitro-L-arginine methyl to ester/kg followed by treatment with lipopolysaccharide displayed a significantly higher gastric retention (74.7% and 80.5%, respectively) as compared to their controls, pretreated with the same doses of the inhibitor and treated with vehicle (40.5% and 38.7%, respectively) and to those pretreated with vehicle and treated with the same toxin. The pretreatment with N(w)-nitro-L-arginine methyl to ester at low dose (1 mg/kg) resulted in a discrete inhibition of the gastric emptying delay induced by lipopolysaccharide. Nevertheless, N(w)-nitro-L-arginine methyl to ester at higher doses (2.5 and 5 mg/kg) induced an enhancement of the lipopolysaccharide effect on gastric emptying, despite not interfering with the gastric emptying per se.
dc.description43
dc.description229-32
dc.languagepor
dc.relationArquivos De Gastroenterologia
dc.relationArq Gastroenterol
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnalysis Of Variance
dc.subjectAnimals
dc.subjectDisease Models, Animal
dc.subjectEnzyme Inhibitors
dc.subjectGastric Emptying
dc.subjectLipopolysaccharides
dc.subjectMale
dc.subjectNg-nitroarginine Methyl Ester
dc.subjectNitric Oxide Synthase
dc.subjectRats
dc.subjectRats, Wistar
dc.title[the Effect Of Bacterial Lipopolysaccharide On The Gastric Emptying Of Rats: A Pretreatment Evaluation Using Nw-nitro-l-arginine Methyl Ester (l-name)].
dc.typeArtículos de revistas


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