Effects Of Experimental Inoculation Of Bovine Respiratory Syncytial Virus In Different Inbred Mice Lineages: Establishment Of A Murine Model For Brsv Infection.

dc.creatorSpilki, Fernando Rosado
dc.creatorAlmeida, Renata Servan
dc.creatorFerreira, Helena Lage
dc.creatorGameiro, Jacy
dc.creatorVerinaud, Liana
dc.creatorArns, Clarice Weis
dc.date2006-Dec
dc.date2015-11-27T13:05:49Z
dc.date2015-11-27T13:05:49Z
dc.date.accessioned2018-03-29T01:03:26Z
dc.date.available2018-03-29T01:03:26Z
dc.identifierVeterinary Microbiology. v. 118, n. 3-4, p. 161-8, 2006-Dec.
dc.identifier0378-1135
dc.identifier10.1016/j.vetmic.2006.07.011
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/16959444
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196928
dc.identifier16959444
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297161
dc.descriptionBovine respiratory syncytial virus (BRSV), a member of the subfamily Pneumovirinae, family Paramyxoviridae, is a major cause of respiratory disorders in young cattle. A number of studies were conducted to validate a reliable animal model for the infection, since BRSV inoculation on the natural host is costly and often unsuccessful. Unfortunately, after inoculation of BRSV in Balb/C mice, viral replication may be detected; however, evident pathological alterations are absent on the experimentally infected animals. In order to establish a mice model that could be used further for preliminary studies of pathological and immunological aspects of BRSV infection, three mice inbred lineages (Balb/C, A/J and C57BL6), possessing different genetic backgrounds, were tested about its susceptibility to the inoculation with BRSV. Animals were inoculated through the nasal and ocular routes and were observed after inoculation. At 7 days post-inoculation (dpi) animals were necropsied and virological (virus isolation and viral nucleic acid amplification) as well as histopathological examinations were performed. A/J and C57BL6 showed interstitial pneumonia, when compared to the Balb/C group. These findings shows that mice may constitute a suitable model for the study of BRSV infections, depending on the mice strain used for experimental inoculations.
dc.description118
dc.description161-8
dc.languageeng
dc.relationVeterinary Microbiology
dc.relationVet. Microbiol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCattle
dc.subjectCattle Diseases
dc.subjectDna, Viral
dc.subjectDisease Models, Animal
dc.subjectImmunohistochemistry
dc.subjectMice
dc.subjectMice, Inbred Balb C
dc.subjectMice, Inbred C57bl
dc.subjectRespiratory Syncytial Virus Infections
dc.subjectRespiratory Syncytial Virus, Bovine
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectSensitivity And Specificity
dc.subjectSpecies Specificity
dc.titleEffects Of Experimental Inoculation Of Bovine Respiratory Syncytial Virus In Different Inbred Mice Lineages: Establishment Of A Murine Model For Brsv Infection.
dc.typeArtículos de revistas


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