Acute Pulmonary Inflammation Induced By Exposure Of The Airways To Staphylococcal Enterotoxin Type B In Rats.

dc.creatorDesouza, Ivani A
dc.creatorFranco-Penteado, Carla F
dc.creatorCamargo, Enilton A
dc.creatorLima, Carmen S P
dc.creatorTeixeira, Simone A
dc.creatorMuscará, Marcelo N
dc.creatorDe Nucci, Gilberto
dc.creatorAntunes, Edson
dc.date2006-Nov
dc.date2015-11-27T13:05:43Z
dc.date2015-11-27T13:05:43Z
dc.date.accessioned2018-03-29T01:03:17Z
dc.date.available2018-03-29T01:03:17Z
dc.identifierToxicology And Applied Pharmacology. v. 217, n. 1, p. 107-13, 2006-Nov.
dc.identifier0041-008X
dc.identifier10.1016/j.taap.2006.07.001
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/16920168
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196893
dc.identifier16920168
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297126
dc.descriptionStaphylococcus aureus is a gram-positive bacterium that produces several enterotoxins, which are responsible for most part of pathological conditions associated to staphylococcal infections, including lung inflammation. This study aimed to investigate the underlying inflammatory mechanisms involved in leukocyte recruitment in rats exposed to staphylococcal enterotoxin B (SEB). Rats were anesthetized with pentobarbital sodium and intratracheally injected with either SEB or sterile phosphate-buffered saline (PBS, 0.4 ml). Airways exposition to SEB (7.5-250 ng/trachea) caused a dose- and time-dependent neutrophil accumulation in BAL fluid, the maximal effects of which were observed at 4 h post-SEB exposure (250 ng/trachea). Eosinophils were virtually absent in BAL fluid, whereas mononuclear cell counts increased only at 24 h post-SEB. Significant elevations of granulocytes in bone marrow (mature and immature forms) and peripheral blood have also been detected. In BAL fluid, marked elevations in the levels of lipid mediators (LTB(4) and PGE(2)) and cytokines (TNF-alpha, IL-6 and IL-10) were observed after SEB instillation. The SEB-induced neutrophil accumulation in BAL fluid was reduced by pretreatment with dexamethasone (0.5 mg/kg), the COX-2 inhibitor celecoxib (3 mg/kg), the selective iNOS inhibitor compound 1400 W (5 mg/kg) and the lipoxygenase inhibitor AA-861 (200 microg/kg). In separate experiments carried out with rat isolated peripheral neutrophils, SEB failed to induce neutrophil adhesion to serum-coated plates and chemotaxis. In conclusion, rat airways exposition to SEB causes a neutrophil-dependent lung inflammation at 4 h as result of the release of proinflammatory (NO, PGE(2), LTB(4), TNF-alpha, IL-6) and anti-inflammatory mediators (IL-10).
dc.description217
dc.description107-13
dc.languageeng
dc.relationToxicology And Applied Pharmacology
dc.relationToxicol. Appl. Pharmacol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAcute Disease
dc.subjectAnimals
dc.subjectAnti-inflammatory Agents
dc.subjectBenzoquinones
dc.subjectBone Marrow
dc.subjectBronchoalveolar Lavage Fluid
dc.subjectChemotaxis, Leukocyte
dc.subjectCytokines
dc.subjectDexamethasone
dc.subjectDinoprostone
dc.subjectDose-response Relationship, Immunologic
dc.subjectEnterotoxins
dc.subjectIntubation, Intratracheal
dc.subjectLeukocyte Count
dc.subjectLeukotriene B4
dc.subjectLipoxygenase Inhibitors
dc.subjectMale
dc.subjectNeutrophils
dc.subjectPneumonia, Staphylococcal
dc.subjectPyrazoles
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSulfonamides
dc.subjectTime Factors
dc.titleAcute Pulmonary Inflammation Induced By Exposure Of The Airways To Staphylococcal Enterotoxin Type B In Rats.
dc.typeArtículos de revistas


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