Biological Activities Of A Lectin From Bothrops Jararacussu Snake Venom.

dc.creatorPanunto, Patrícia C
dc.creatorda Silva, Maura A
dc.creatorLinardi, Alessandra
dc.creatorBuzin, Marta P
dc.creatorMelo, Silvia E S F C
dc.creatorMello, Sueli M
dc.creatorPrado-Franceschi, Julia
dc.creatorHyslop, Stephen
dc.date2006-Jan
dc.date2015-11-27T13:05:29Z
dc.date2015-11-27T13:05:29Z
dc.date.accessioned2018-03-29T01:02:52Z
dc.date.available2018-03-29T01:02:52Z
dc.identifierToxicon : Official Journal Of The International Society On Toxinology. v. 47, n. 1, p. 21-31, 2006-Jan.
dc.identifier0041-0101
dc.identifier10.1016/j.toxicon.2005.08.012
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/16309723
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196784
dc.identifier16309723
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1297017
dc.descriptionSnake venoms contain saccharide-binding lectins. In this work, we examined the biological activities of a lectin (BjcuL) purified from Bothrops jararacussu snake venom by chromatography on non-derivatized Sepharose 4B and Sephacryl S-200 HR. The protein, a homodimer with subunits of 14.5 kDa, gave a single immunoprecipitin line in immunoelectrophoresis and cross-reacted in ELISA with antivenoms raised against Bothrops spp. (lanceheads), Micrurus spp. (coral snakes), Crotalus durissus terrificus (South American rattlesnake), and arthropod (Loxosceles gaucho, Phoneutria nigriventer and Tityus serrulatus) venoms. BjcuL agglutinated human formaldehyde-fixed erythrocytes at > or = 100 ng/ml and was inhibited by lactose and EDTA (> or = 2 mM) and high concentrations (> 100 mM) of glucose and sucrose, but not by N-acetylglucosamine. BjcuL had no direct hemolytic activity and was devoid of esterase, PLA2 and proteolytic activities. The lectin (up to 200 microg/ml) did not aggregate human platelet-rich plasma (PRP) or washed platelets (WP), nor did it alter the aggregation induced by ADP in PRP or by thrombin in WP. When injected into mouse hind paws, BjcuL (10-100 microg/paw) caused edema and increased vascular permeability, with a maximum effect after 1h that persisted for up to 6 h (edema) or gradually decreased after the peak interval (vascular permeability). No hemorrhage was observed in BjcuL-injected paws. In anesthetized rats, B. jararacussu venom (200 microg/kg, i.v.) produced sustained hypotension (maximum decrease of approximately 60%) whereas a similar dose of BjcuL decreased the blood pressure by approximately 15%, with a rapid return to the resting level.
dc.description47
dc.description21-31
dc.languageeng
dc.relationToxicon : Official Journal Of The International Society On Toxinology
dc.relationToxicon
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBlood Platelets
dc.subjectBlood Pressure
dc.subjectBlood Vessels
dc.subjectBothrops
dc.subjectCapillary Permeability
dc.subjectDose-response Relationship, Drug
dc.subjectHumans
dc.subjectLectins
dc.subjectMice
dc.subjectMolecular Weight
dc.subjectPlatelet Aggregation Inhibitors
dc.subjectRats
dc.subjectSnake Venoms
dc.titleBiological Activities Of A Lectin From Bothrops Jararacussu Snake Venom.
dc.typeArtículos de revistas


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