Effects Of Veratrine And Veratridine On Oxygen Consumption And Electrical Membrane Potential Of Isolated Rat Skeletal Muscle And Liver Mitochondria.

dc.creatorSilva Freitas, Erika Maria
dc.creatorFagian, Márcia M
dc.creatorda Cruz Höfling, Maria Alice
dc.date2006-Jun
dc.date2015-11-27T13:05:21Z
dc.date2015-11-27T13:05:21Z
dc.date.accessioned2018-03-29T01:02:39Z
dc.date.available2018-03-29T01:02:39Z
dc.identifierToxicon : Official Journal Of The International Society On Toxinology. v. 47, n. 7, p. 780-7, 2006-Jun.
dc.identifier0041-0101
dc.identifier10.1016/j.toxicon.2006.02.009
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/16626771
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196727
dc.identifier16626771
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296960
dc.descriptionWe have previously shown that veratrine, a mixture of alkaloids known as Veratrum alkaloids, produces skeletal muscle toxicity, and there is evidence that veratrine interferes with the energetics of various systems, including cardiomyocytes and synaptosomes. In this work, we explored the effects of veratrine and veratridine, a component of this mixture, in rat skeletal muscle mitochondria and compared the results with those seen in liver mitochondria. Veratrine and veratridine alkaloids caused a significant concentration-dependent decrease in the rate of state 3 respiration, respiratory control (RCR) and ADP/O ratios in isolated rat skeletal muscle mitochondria (RMM), but not in rat liver mitochondria (RLM) supported by either NADH-linked substrates or succinate. The oxygen consumption experiments showed that RMM were more susceptible to the toxic action of Veratrum alkaloids than RLM. The addition of veratrine (250 microg/ml) to RMM caused dissipation of the mitochondrial electrical membrane potential during succinate oxidation, but this effect was totally reversed by adding ATP. These results indicate that there are chemical- and tissue-specific toxic effects of veratrine and veratridine on mitochondrial respiratory chain complexes. Identification of the specific respiratory chain targets involved should provide a better understanding of the molecular mechanisms of the toxicity of these agents.
dc.description47
dc.description780-7
dc.languageeng
dc.relationToxicon : Official Journal Of The International Society On Toxinology
dc.relationToxicon
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectDose-response Relationship, Drug
dc.subjectFemale
dc.subjectMale
dc.subjectMembrane Potentials
dc.subjectMitochondria, Liver
dc.subjectMitochondria, Muscle
dc.subjectMuscle, Skeletal
dc.subjectOxygen Consumption
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectVeratridine
dc.subjectVeratrine
dc.titleEffects Of Veratrine And Veratridine On Oxygen Consumption And Electrical Membrane Potential Of Isolated Rat Skeletal Muscle And Liver Mitochondria.
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución