Leishmanicidal Activity Of Primary S-nitrosothiols Against Leishmania Major And Leishmania Amazonensis: Implications For The Treatment Of Cutaneous Leishmaniasis.

dc.creatorde Souza, Gabriela Freitas Pereira
dc.creatorYokoyama-Yasunaka, Jenicer K U
dc.creatorSeabra, Amedea Barozzi
dc.creatorMiguel, Danilo Ciccone
dc.creatorde Oliveira, Marcelo Ganzarolli
dc.creatorUliana, Silvia Reni B
dc.date2006-Nov
dc.date2015-11-27T13:05:14Z
dc.date2015-11-27T13:05:14Z
dc.date.accessioned2018-03-29T01:02:26Z
dc.date.available2018-03-29T01:02:26Z
dc.identifierNitric Oxide : Biology And Chemistry / Official Journal Of The Nitric Oxide Society. v. 15, n. 3, p. 209-16, 2006-Nov.
dc.identifier1089-8603
dc.identifier10.1016/j.niox.2006.01.011
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/16527502
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196673
dc.identifier16527502
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296906
dc.descriptionNitric oxide (NO) is considered a key molecule in the defense against intracellular pathogens, particularly Leishmania. The expression of inducible nitric oxide synthase and consequent production of NO by infected macrophages has been shown to correlate with leishmaniasis resistance in the murine model as well as in human patients. Nitric oxide donors have been used successfully in the treatment of cutaneous leishmaniasis in humans, although their mechanisms of action are not fully understood. In the present work, the dose-dependent cytotoxic effects of the NO-donors S-nitroso-N-acetyl-l-cysteine (SNAC) and S-nitrosoglutathione (GSNO) against Leishmania were evaluated. GSNO inhibited the growth of Leishmania major and Leishmania amazonensis with in vitro 50% inhibitory concentrations (IC(50)) of 68.8+/-22.86 and 68.9+/-7.9 micromol L(-1), respectively. The IC(50) for SNAC against L. major and L. amazonensis were, respectively, 54.6+/-8.3 and 181.6+/-12.5 micromol L(-1). The leishmanicidal activity of GSNO, but not of SNAC, was reversed by ascorbic acid (AA) and dithiothreitol (DTT), suggesting that the mechanism of action of GSNO is related to the transnitrosation of parasite proteins. These results demonstrate that SNAC and GSNO have leishmanicidal activity, and are thus potential therapeutic agents against cutaneous leishmaniasis.
dc.description15
dc.description209-16
dc.languageeng
dc.relationNitric Oxide : Biology And Chemistry / Official Journal Of The Nitric Oxide Society
dc.relationNitric Oxide
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAcetylcysteine
dc.subjectAnimals
dc.subjectCell Survival
dc.subjectHumans
dc.subjectLeishmania Major
dc.subjectLeishmaniasis, Cutaneous
dc.subjectNitric Oxide
dc.subjectNitric Oxide Donors
dc.subjectS-nitrosoglutathione
dc.titleLeishmanicidal Activity Of Primary S-nitrosothiols Against Leishmania Major And Leishmania Amazonensis: Implications For The Treatment Of Cutaneous Leishmaniasis.
dc.typeArtículos de revistas


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