[critical And Methodological Analyses On The Determination Of Oxygen And Nitrogen Reactive Species In Skeletal Muscle Cells During Contractions].

dc.creatorSilveira, Leonardo R
dc.date2004-Dec
dc.date2015-11-27T13:02:51Z
dc.date2015-11-27T13:02:51Z
dc.date.accessioned2018-03-29T01:02:13Z
dc.date.available2018-03-29T01:02:13Z
dc.identifierArquivos Brasileiros De Endocrinologia E Metabologia. v. 48, n. 6, p. 812-22, 2004-Dec.
dc.identifier0004-2730
dc.identifier/S0004-27302004000600006
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15761554
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196620
dc.identifier15761554
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296853
dc.descriptionAn excessive level of these species may be harmful as it may overcome the antioxidant capacity. Most evidences for ROS and NO formation in muscle cell are indirect whereas direct evidences are scarce. The present review suggests as an alternative method either fluorescent DCFH, DAF-2 or fluorescamine toward H2O2, NO and O2*- measurements, respectively in muscle cell. In addition, it was concluded that: 1) the indirect measurements of ROS and NO in blood sample do not reflect oxidative attack occurred in muscle cells during contractions; 2) isolated muscles and homogenates may contain a large amount of endothelial cells, red blood cells and leukocytes which may generate ROS and NO; 3) the fluorescent DCFH-DA/DCFH, DAF-2-DA/DAF-2 and fluorescamine are sensitive in detection of H2O2, NO and O2.- respectively, in muscle cell during contractions; 4) muscle cell culture and isolated muscle fiber are indicated as experimental models to study ROS and NO formation in muscular tissue.
dc.description48
dc.description812-22
dc.languagepor
dc.relationArquivos Brasileiros De Endocrinologia E Metabologia
dc.relationArq Bras Endocrinol Metabol
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectHumans
dc.subjectMuscle Contraction
dc.subjectMuscle, Skeletal
dc.subjectReactive Nitrogen Species
dc.subjectReactive Oxygen Species
dc.title[critical And Methodological Analyses On The Determination Of Oxygen And Nitrogen Reactive Species In Skeletal Muscle Cells During Contractions].
dc.typeArtículos de revistas


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