Search For Herpesvirus 1 And 2 By In Situ Hybridization In Tonsils And Adenoids.

dc.creatorVassallo, José
dc.creatorCamargo, Leandro Azevedo de
dc.creatorChagas, Cristiano Aparecido
dc.creatorPinto, Glauce Aparecida
dc.creatorEndo, Luiza Hayashi
dc.date2005-Mar
dc.date2015-11-27T13:02:49Z
dc.date2015-11-27T13:02:49Z
dc.date.accessioned2018-03-29T01:02:09Z
dc.date.available2018-03-29T01:02:09Z
dc.identifierInternational Journal Of Pediatric Otorhinolaryngology. v. 69, n. 3, p. 345-9, 2005-Mar.
dc.identifier0165-5876
dc.identifier10.1016/j.ijporl.2004.10.006
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15733593
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196599
dc.identifier15733593
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296832
dc.descriptionHerpes simplex virus (HSV) has been described as cause of acute tonsillitis. It has also been found in nasopharyngeal florid lymphoid infiltrate, mostly composed of CD4+, CD56+ T-cells, simulating lymphoma. In spite of its widespread prevalence in latent form, to the best of our knowledge no study is available on in situ detection of HSV in chronically hyperplastic nasopharyngeal lymphoid tissue. The purpose of the present study was to search for the presence of HSV 1 and 2 in 21 adenoids and 15 tonsils from children (2-12 years of age) in which these organs had been surgically removed due to hypertrophy. Paraffin wax-embedded sections from the 36 cases were submitted to the in situ hybridization technique, using the biotinilated probe to Herpes simplex virus 1 and 2 (Pan Path, Amsterdam) and the Rembrandt Universal DISH & HRP Detection Kit (Pan Path, Amsterdam). Positive control consisted of a previously tested Herpes infected lung. In none of the 36 cases studied were positive nuclei detected in adenoid and tonsils, either in lymphoid, in stroma or in epithelial cells, as those seen in the positive control. HSV does not seem to be implied in tonsil or adenoid chronic lymphoid hyperplasia. These organs do not seem to harbor the virus latently, or the amount of virus is too low to be detected without amplification methods.
dc.description69
dc.description345-9
dc.languageeng
dc.relationInternational Journal Of Pediatric Otorhinolaryngology
dc.relationInt. J. Pediatr. Otorhinolaryngol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAdenoids
dc.subjectAntigens, Cd4
dc.subjectAntigens, Cd56
dc.subjectChild
dc.subjectChild, Preschool
dc.subjectFemale
dc.subjectHerpes Simplex
dc.subjectHerpesvirus 1, Human
dc.subjectHerpesvirus 2, Human
dc.subjectHumans
dc.subjectHyperplasia
dc.subjectIn Situ Hybridization
dc.subjectLymphoid Tissue
dc.subjectMale
dc.subjectNasopharynx
dc.subjectTonsillitis
dc.titleSearch For Herpesvirus 1 And 2 By In Situ Hybridization In Tonsils And Adenoids.
dc.typeArtículos de revistas


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