Aqueous Extraction Of Recombinant Human Proinsulin From Transgenic Maize Endosperm.

dc.creatorFarinas, Cristiane S
dc.creatorLeite, Adilson
dc.creatorMiranda, Everson A
dc.date
dc.date2015-11-27T13:02:29Z
dc.date2015-11-27T13:02:29Z
dc.date.accessioned2018-03-29T01:01:33Z
dc.date.available2018-03-29T01:01:33Z
dc.identifierBiotechnology Progress. v. 21, n. 5, p. 1466-71
dc.identifier8756-7938
dc.identifier10.1021/bp050103r
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/16209552
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196445
dc.identifier16209552
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296678
dc.descriptionDifferent plant species have been used as systems to produce recombinant proteins. Maize is a crop considered to have a large potential to produce high levels of recombinant proteins and is the host for the recombinant proteins from plants currently available on the market. In the development of a plant system to produce a recombinant proteins it is important to consider the costs related to downstream processing. Also, the steps necessary to achieve the protein purity required will be highly influenced by the quality of the extract obtained. In this study, we analyzed aqueous extracts from the endosperm of transgenic maize expressing recombinant human proinsulin (rhProinsulin). A study of the effects of the variables pH and ionic strength on the extraction efficiency was carried out using experimental design and response surface methodology. Besides the concentration of the recombinant protein, the characteristics of the extracts were evaluated in terms of concentration of native components (proteins, carbohydrates, and phenolic compounds) and extract filterability. The highest rhProinsulin concentration (97.33 ng/mL) was found with a 200 mM NaCl pH 10.0 extraction solution. Under this experimental condition the concentrations of total soluble proteins, carbohydrates, and phenolics were 2.01 mg/mL, 2.21 mg/mL, and 0.11 mmol/L, respectively.
dc.description21
dc.description1466-71
dc.languageeng
dc.relationBiotechnology Progress
dc.relationBiotechnol. Prog.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectChemical Fractionation
dc.subjectCombinatorial Chemistry Techniques
dc.subjectHumans
dc.subjectPlants, Genetically Modified
dc.subjectProinsulin
dc.subjectProtein Engineering
dc.subjectSeeds
dc.subjectSolutions
dc.subjectUltrafiltration
dc.subjectWater
dc.subjectZea Mays
dc.titleAqueous Extraction Of Recombinant Human Proinsulin From Transgenic Maize Endosperm.
dc.typeArtículos de revistas


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