Detection Of Brazilian Bovine Respiratory Syncytial Virus Strain By A Reverse Transcriptase-nested-polymerase Chain Reaction In Experimentally Infected Calves.

dc.creatorAlmeida, Renata S
dc.creatorSpilki, Fernando R
dc.creatorRoehe, Paulo M
dc.creatorArns, Clarice W
dc.date2005-Jan
dc.date2015-11-27T13:02:00Z
dc.date2015-11-27T13:02:00Z
dc.date.accessioned2018-03-29T01:00:38Z
dc.date.available2018-03-29T01:00:38Z
dc.identifierVeterinary Microbiology. v. 105, n. 2, p. 131-5, 2005-Jan.
dc.identifier0378-1135
dc.identifier10.1016/j.vetmic.2004.11.004
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15627524
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196216
dc.identifier15627524
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296449
dc.descriptionA reverse transcriptase (RT)-nested-polymerase chain reaction (PCR) was standardised to detect bovine respiratory syncytial virus (BRSV), using a Brazilian isolate, in three experimentally infected calves. This followed initial tests in infected chicken embryo related (CER) cells. One animal had lesions, characterized by interstitial multifocal pneumonia, severe interstitial and subpleural emphysema, and lung consolidated areas. Lung and tracheal tissues collected 6 days after infection were analysed by RT-nested-PCR. Primers, specific for the BRSV G and F glycoproteins genes, yielded amplification fragments of 371 and 481 bp, respectively, from the RNA of the cell-propagated virus. Using RNA extracted from organs of infected calves, RT-nested-PCR amplified the fragment of the G gene in all tracheal samples, but in only two of three lung samples analysed. These results suggest that RT-nested-PCR could be a promising assay for diagnosis and epidemiological analysis of BRSV in Brazil.
dc.description105
dc.description131-5
dc.languageeng
dc.relationVeterinary Microbiology
dc.relationVet. Microbiol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBrazil
dc.subjectCattle
dc.subjectCattle Diseases
dc.subjectChick Embryo
dc.subjectLung
dc.subjectRna, Viral
dc.subjectRespiratory Syncytial Virus Infections
dc.subjectRespiratory Syncytial Virus, Bovine
dc.subjectRespiratory Tract Diseases
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectTrachea
dc.subjectViral Proteins
dc.titleDetection Of Brazilian Bovine Respiratory Syncytial Virus Strain By A Reverse Transcriptase-nested-polymerase Chain Reaction In Experimentally Infected Calves.
dc.typeArtículos de revistas


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