Rapid Method For Evaluating Reversed-phase High-performance Liquid Chromatography Column Stability.

dc.creatorFonseca, Dania A
dc.creatorGutiérrez, Humberto R
dc.creatorCollins, Kenneth E
dc.creatorCollins, Carol H
dc.date2004-Mar
dc.date2015-11-27T12:58:49Z
dc.date2015-11-27T12:58:49Z
dc.date.accessioned2018-03-29T01:00:28Z
dc.date.available2018-03-29T01:00:28Z
dc.identifierJournal Of Chromatography. A. v. 1030, n. 1-2, p. 149-55, 2004-Mar.
dc.identifier0021-9673
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15043264
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196170
dc.identifier15043264
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296403
dc.descriptionA procedure is presented for the rapid evaluation of HPLC stationary phase stability at pH 8.4 or 10.1 using a temperature of 60 degrees C. Mobile phase (MeOH-0.1 mol l(-1) aqueous NaHCO3, 50:50, v/v) is continuously passed through the column with periodic injections of a test solution until the several chromatographic parameters of the resulting chromatograms are degraded. The tests were applied to several commercial and laboratory-made stationary phases. After degradation two of these phases, one commercial and one laboratory-made, were examined by elemental analysis and scanning electron microscopy to elucidate the degradation process.
dc.description1030
dc.description149-55
dc.languageeng
dc.relationJournal Of Chromatography. A
dc.relationJ Chromatogr A
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectChromatography, High Pressure Liquid
dc.subjectHydrogen-ion Concentration
dc.titleRapid Method For Evaluating Reversed-phase High-performance Liquid Chromatography Column Stability.
dc.typeArtículos de revistas


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