Protein Detection In Spermatids And Spermatozoa Of The Butterfly Euptoieta Hegesia (lepidoptera).

dc.creatorMancini, Karina
dc.creatorDolder, Heidi
dc.date2004-Dec
dc.date2015-11-27T12:58:39Z
dc.date2015-11-27T12:58:39Z
dc.date.accessioned2018-03-29T01:00:05Z
dc.date.available2018-03-29T01:00:05Z
dc.identifierBiocell : Official Journal Of The Sociedades Latinoamericanas De Microscopía Electronica ... Et. Al. v. 28, n. 3, p. 299-310, 2004-Dec.
dc.identifier0327-9545
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15633453
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196071
dc.identifier15633453
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296304
dc.descriptionThis study was undertaken to detect protein components in both sperm types of the butterfly Euptoieta hegesia. These spermatozoa possess complex extracellular structures for which the composition and functional significance are still unclear. In the apyrene sperm head, the proteic cap presented an external ring and an internal dense content; basic proteins were detected only in external portions. In the tail, the paracrystalline core of mitochondrial derivatives and the axoneme are rich in proteins. The extratesticular spermatozoa are covered by a proteic coat, which presented two distinct layers. In eupyrene spermatozoa, acrosome and nucleus were negatively stained, probably because of their high compaction. In the tail, there is no paracrystalline core and the axoneme presented a very specific reaction for basic proteins. The lacinate and reticular appendages are composed of cylindrical sub-units and presented a light reaction to E-PTA and a strong reaction to tannic acid. A complex proteic coat also covers the extratesticular spermatozoa. We found similarities between both extratesticular coats, indicating a possible common origin. Both spermatozoon types are rich in proteins, especially the eupyrene appendages and the extratesticular coats. We believe that both coats are related to the sperm maturation and capacitation processes.
dc.description28
dc.description299-310
dc.languageeng
dc.relationBiocell : Official Journal Of The Sociedades Latinoamericanas De Microscopía Electronica ... Et. Al
dc.relationBiocell
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAcrosome
dc.subjectAnimals
dc.subjectButterflies
dc.subjectCell Nucleus
dc.subjectCentrioles
dc.subjectInsect Proteins
dc.subjectMale
dc.subjectMicroscopy, Electron, Transmission
dc.subjectSeminal Vesicles
dc.subjectSperm Tail
dc.subjectSpermatids
dc.subjectSpermatozoa
dc.subjectStaining And Labeling
dc.subjectTestis
dc.subjectVas Deferens
dc.titleProtein Detection In Spermatids And Spermatozoa Of The Butterfly Euptoieta Hegesia (lepidoptera).
dc.typeArtículos de revistas


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