Hypoxia Modulates Expression Of The 70-kd Heat Shock Protein And Reduces Leishmania Infection In Macrophages.

dc.creatorDegrossoli, Adriana
dc.creatorColhone, Marcelle Carolina
dc.creatorArrais-Silva, Wagner Welber
dc.creatorGiorgio, Selma
dc.date
dc.date2015-11-27T12:58:37Z
dc.date2015-11-27T12:58:37Z
dc.date.accessioned2018-03-29T01:00:00Z
dc.date.available2018-03-29T01:00:00Z
dc.identifierJournal Of Biomedical Science. v. 11, n. 6, p. 847-54
dc.identifier1021-7770
dc.identifier10.1159/000081832
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15591782
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196051
dc.identifier15591782
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296284
dc.descriptionHypoxia, a microenvironmental factor present in diseased tissues, has been recognized as a specific metabolic stimulus or a signal of cellular response. Experimental hypoxia has been reported to induce adaptation in macrophages such as differential migration, elevation of proinflammatory cytokines and glycolytic enzyme activities, and decreased phagocytosis of inert particles. In this study we demonstrate that although exposure to hypoxia (5% O2, 5% CO2, and balanced N2) did not change macrophage viability, or 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cleavage and proliferation, it significantly reduced expression of the 70-kD heat shock protein (HSP70), which was restored to prehypoxia levels after reoxygenation. The influence of low oxygen tension on macrophage functional activity was also studied, i.e. the ability of these cells to maintain or resist infection by a microorganism. We demonstrate that macrophages from two different sources (a murine cell line and primary cells) exposed to hypoxia were efficiently infected with Leishmania amazonensis, but after 24 h showed a reduction in the percentage of infected cells and of the number of intracellular parasites per macrophage, indicating that hypoxia induced macrophages to kill the intracellular parasites. These results support the notion that hypoxia, a microenvironmental factor, can modulate macrophage protein expression and functional activity.
dc.description11
dc.description847-54
dc.languageeng
dc.relationJournal Of Biomedical Science
dc.relationJ. Biomed. Sci.
dc.rightsaberto
dc.rights2004 National Science Council, ROC and S. Karger AG, Basel
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAnoxia
dc.subjectBlotting, Western
dc.subjectCell Line
dc.subjectCell Movement
dc.subjectCell Survival
dc.subjectColoring Agents
dc.subjectGlycolysis
dc.subjectHsp70 Heat-shock Proteins
dc.subjectImmunoblotting
dc.subjectLeishmania
dc.subjectLeishmaniasis
dc.subjectMacrophages
dc.subjectMice
dc.subjectOxygen
dc.subjectReactive Oxygen Species
dc.subjectTetrazolium Salts
dc.subjectThiazoles
dc.subjectTime Factors
dc.titleHypoxia Modulates Expression Of The 70-kd Heat Shock Protein And Reduces Leishmania Infection In Macrophages.
dc.typeArtículos de revistas


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