Participation Of Prolactin Receptors And Phosphatidylinositol 3-kinase And Map Kinase Pathways In The Increase In Pancreatic Islet Mass And Sensitivity To Glucose During Pregnancy.

dc.creatorAmaral, Maria E C
dc.creatorCunha, Daniel A
dc.creatorAnhê, Gabriel F
dc.creatorUeno, Mirian
dc.creatorCarneiro, Everardo M
dc.creatorVelloso, Lício A
dc.creatorBordin, Silvana
dc.creatorBoschero, Antonio C
dc.date2004-Dec
dc.date2015-11-27T12:58:37Z
dc.date2015-11-27T12:58:37Z
dc.date.accessioned2018-03-29T01:00:00Z
dc.date.available2018-03-29T01:00:00Z
dc.identifierThe Journal Of Endocrinology. v. 183, n. 3, p. 469-76, 2004-Dec.
dc.identifier0022-0795
dc.identifier10.1677/joe.1.05547
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15590973
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196050
dc.identifier15590973
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296283
dc.descriptionProlactin (PRL) exerts its biological effects mainly by activating the Janus kinase/signal transducer and activator of transcription 5 (JAK/STAT5) signaling pathway. We have recently demonstrated that PRL also stimulates the insulin receptor substrates/phosphatidylinositol 3-kinase (IRSs/PI3K) and SH2-plekstrin homology domain (SHC)/ERK pathways in islets of neonatal rats. In the present study, we investigated the involvement of the PI3K and MAP kinase (MAPK) cascades in islet development and growth in pregnant rats. The protein expression of AKT1, p70S6K and SHC was higher in islets from pregnant compared with control rats. Higher basal levels of tyrosine phosphorylation were found in classic transducers of insulin cell signaling (IRS1, IRS2 and SHC). Increased levels of threonine/tyrosine phosphorylation of ERK1/2 and serine phosphorylation of AKT and p70S6K were also detected. To assess the participation of PRL in these phenomena, pregnant and control rats were treated with an antisense oligonucleotide to reduce the expression of the PRL receptor (PRLR). Phosphorylation of AKT was reduced in islets from pregnant and control rats, whereas p70S6K protein levels were reduced only in islets from treated pregnant rats. Finally, glucose-induced insulin secretion was reduced in islets from pregnant but not from control rats treated with the PRLR antisense oligonucleotide. In conclusion, downstream proteins of the PI3K (AKT and p70S6K) and MAPK (SHC and ERK1/2) cascades are regulated by PRL signaling in islets from pregnant rats. These findings indicate that these pathways participate in the increase in islet mass and the sensitivity to glucose during pregnancy.
dc.description183
dc.description469-76
dc.languageeng
dc.relationThe Journal Of Endocrinology
dc.relationJ. Endocrinol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCells, Cultured
dc.subjectFemale
dc.subjectGlucose
dc.subjectInsulin
dc.subjectIslets Of Langerhans
dc.subjectMap Kinase Signaling System
dc.subjectOligonucleotides, Antisense
dc.subjectPhosphatidylinositol 3-kinases
dc.subjectPhosphorylation
dc.subjectPregnancy
dc.subjectProtein-serine-threonine Kinases
dc.subjectProto-oncogene Proteins
dc.subjectProto-oncogene Proteins C-akt
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReceptors, Prolactin
dc.subjectRibosomal Protein S6 Kinases, 70-kda
dc.titleParticipation Of Prolactin Receptors And Phosphatidylinositol 3-kinase And Map Kinase Pathways In The Increase In Pancreatic Islet Mass And Sensitivity To Glucose During Pregnancy.
dc.typeArtículos de revistas


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