Rarity Of Dna Sequence Alterations In The Promoter Region Of The Human Androgen Receptor Gene.

dc.creatorCabral, D F
dc.creatorSantos, A
dc.creatorRibeiro, M L
dc.creatorMesquita, J C
dc.creatorCarvalho-Salles, A B
dc.creatorHackel, C
dc.date2004-Dec
dc.date2015-11-27T12:58:36Z
dc.date2015-11-27T12:58:36Z
dc.date.accessioned2018-03-29T00:59:57Z
dc.date.available2018-03-29T00:59:57Z
dc.identifierBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 37, n. 12, p. 1789-94, 2004-Dec.
dc.identifier0100-879X
dc.identifier/S0100-879X2004001200004
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15558185
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196038
dc.identifier15558185
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296271
dc.descriptionThe human androgen receptor (AR) gene promoter lies in a GC-rich region containing two principal sites of transcription initiation and a putative Sp1 protein-binding site, without typical TATA and CAAT boxes. It has been suggested that mutations within the 5'untranslated region (5'UTR) may contribute to the development of prostate cancer by changing the rates of gene transcription and/or translation. In order to investigate this question, the aim of the present study was to search for the presence of mutations or polymorphisms at the AR-5'UTR in 92 prostate cancer patients, where histological diagnosis of adenocarcinoma was established in specimens obtained from transurethral resection or after prostatectomy. The AR-5'UTR was amplified by PCR from genomic DNA samples of the patients and of 100 healthy male blood donors, included as controls. Conformation-sensitive gel electrophoresis was used for DNA sequence alteration screening. Only one band shift was detected in one individual from the blood donor group. Sequencing revealed a new single nucleotide deletion (T) in the most conserved portion of the promoter region at position +36 downstream from the transcription initiation site I. Although the effect of this specific mutation remains unknown, its rarity reveals the high degree of sequence conservation of the human androgen promoter region. Moreover, the absence of detectable variation within the critical 5'UTR in prostate cancer patients indicates a low probability of its involvement in prostate cancer etiology.
dc.description37
dc.description1789-94
dc.languageeng
dc.relationBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]
dc.relationBraz. J. Med. Biol. Res.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subject5' Untranslated Regions
dc.subjectAdenocarcinoma
dc.subjectAdolescent
dc.subjectAdult
dc.subjectCase-control Studies
dc.subjectGenetic Markers
dc.subjectHumans
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectMutation
dc.subjectPolymorphism, Genetic
dc.subjectPromoter Regions, Genetic
dc.subjectProstatic Neoplasms
dc.subjectReceptors, Androgen
dc.subjectSequence Analysis, Dna
dc.titleRarity Of Dna Sequence Alterations In The Promoter Region Of The Human Androgen Receptor Gene.
dc.typeArtículos de revistas


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