Fluoro-jade, But Not Fluoro-jade B, Stains Non-degenerating Cells In Brain And Retina Of Embryonic And Neonatal Rats.

dc.creatorFernandes, Anna M A P
dc.creatorMaurer-Morelli, Cláudia V
dc.creatorCampos, Cláudia B L
dc.creatorMello, Maria L S
dc.creatorCastilho, Roger F
dc.creatorLangone, Francesco
dc.date2004-Dec
dc.date2015-11-27T12:58:34Z
dc.date2015-11-27T12:58:34Z
dc.date.accessioned2018-03-29T00:59:51Z
dc.date.available2018-03-29T00:59:51Z
dc.identifierBrain Research. v. 1029, n. 1, p. 24-33, 2004-Dec.
dc.identifier0006-8993
dc.identifier10.1016/j.brainres.2004.09.036
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15533312
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196016
dc.identifier15533312
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296249
dc.descriptionFluoro-Jade (FJ) and Fluoro-Jade B (FJB) are fluorescein derivatives currently used to stain brain cells under degeneration. In this study, we investigated the FJ staining of nondegenerating cells in embryonic and neonatal rat brain and retina. In embryonic rat brain (embryonic day 15; E15), very intense staining of cells was observed. The number of FJ-stained cells and the intensity of staining decreased with increasing in animal age, being almost absent by postnatal day 16 (P16). Only a few cells in neonatal rat brain were in the process of cell death, as verified by the TUNEL technique. The FJ-stained cells in neonatal brain were positive for the neuronal marker neuronal nuclei antigen (NeuN). In retina, FJ stained mainly cells from the ganglion cell layer at P2 and the neuroblastic layer at P2 and P6. In contrast to FJ, FJB did not stain nondegenerating cells in embryonic and neonatal rats. These results show that in addition to staining degenerating brain cells, FJ also stains nondegenerating central nervous system cells in embryonic and neonatal stages.
dc.description1029
dc.description24-33
dc.languageeng
dc.relationBrain Research
dc.relationBrain Res.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBrain
dc.subjectBrain Chemistry
dc.subjectCell Survival
dc.subjectFemale
dc.subjectFluoresceins
dc.subjectFluorescent Dyes
dc.subjectIn Situ Nick-end Labeling
dc.subjectNerve Degeneration
dc.subjectOrganic Chemicals
dc.subjectOrganogenesis
dc.subjectPregnancy
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectRetina
dc.subjectStaining And Labeling
dc.titleFluoro-jade, But Not Fluoro-jade B, Stains Non-degenerating Cells In Brain And Retina Of Embryonic And Neonatal Rats.
dc.typeArtículos de revistas


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