Evaluation Of An In-house Specific Immunoglobulin G (igg) Avidity Elisa For Distinguishing Recent Primary From Long-term Human Cytomegalovirus (hcmv) Infection.

dc.creatorde Souza, Silmara
dc.creatorBonon, Sandra H A
dc.creatorCosta, Sandra C B
dc.creatorRossi, Cláudio L
dc.date
dc.date2015-11-27T12:58:32Z
dc.date2015-11-27T12:58:32Z
dc.date.accessioned2018-03-29T00:59:47Z
dc.date.available2018-03-29T00:59:47Z
dc.identifierRevista Do Instituto De Medicina Tropical De São Paulo. v. 45, n. 6, p. 323-6
dc.identifier0036-4665
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/14762632
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195998
dc.identifier14762632
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296231
dc.descriptionThis article describes the standardization and evaluation of an in-house specific IgG avidity ELISA for distinguishing recent primary from long-term human cytomegalovirus (HCMV) infection. The test was standardized with the commercial kit ETI-CYTOK G Plus (Sorin Biomedica, Italy) using 8 M urea in phosphate-buffered saline to dissociate low-avidity antibodies after the antigen-antibody interaction. The performance of the in-house assay was compared to that of the commercial automated VIDAS CMV IgG avidity test (bioM rieux, France). Forty-nine sera, 24 from patients with a recent primary HCMV infection and 25 from patients with a long-term HCMV infection and a sustained persistence of specific IgM antibodies, were tested. Similar results were obtained with the two avidity methods. All 24 sera from patients with recently acquired infection had avidity indices compatible with acute HCMV infection by the VIDAS method, whereas with the in-house method, one serum sample had an equivocal result. In the 25 sera from patients with long-term infection, identical results were obtained with the two methods, with only one serum sample having an incompatible value. These findings suggest that our in-house avidity test could be a potentially useful tool for the immunodiagnosis of HCMV infection.
dc.description45
dc.description323-6
dc.languageeng
dc.relationRevista Do Instituto De Medicina Tropical De São Paulo
dc.relationRev. Inst. Med. Trop. Sao Paulo
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAntibodies, Viral
dc.subjectAntibody Affinity
dc.subjectCytomegalovirus
dc.subjectCytomegalovirus Infections
dc.subjectEnzyme-linked Immunosorbent Assay
dc.subjectFemale
dc.subjectHumans
dc.subjectImmunoglobulin G
dc.subjectReagent Kits, Diagnostic
dc.titleEvaluation Of An In-house Specific Immunoglobulin G (igg) Avidity Elisa For Distinguishing Recent Primary From Long-term Human Cytomegalovirus (hcmv) Infection.
dc.typeArtículos de revistas


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