Respiration, Oxidative Phosphorylation, And Uncoupling Protein In Candida Albicans.

dc.creatorCavalheiro, R A
dc.creatorFortes, F
dc.creatorBorecký, J
dc.creatorFaustinoni, V C
dc.creatorSchreiber, A Z
dc.creatorVercesi, A E
dc.date2004-Oct
dc.date2015-11-27T12:58:28Z
dc.date2015-11-27T12:58:28Z
dc.date.accessioned2018-03-29T00:59:39Z
dc.date.available2018-03-29T00:59:39Z
dc.identifierBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 37, n. 10, p. 1455-61, 2004-Oct.
dc.identifier0100-879X
dc.identifier/S0100-879X2004001000003
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15448865
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195962
dc.identifier15448865
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296195
dc.descriptionThe respiration, membrane potential (Deltapsi), and oxidative phosphorylation of mitochondria in situ were determined in spheroplasts obtained from Candida albicans control strain ATCC 90028 by lyticase treatment. Mitochondria in situ were able to phosphorylate externally added ADP (200 microM) in the presence of 0.05% BSA. Mitochondria in situ generated and sustained stable mitochondrial Deltapsi respiring on 5 mM NAD-linked substrates, 5 mM succinate, or 100 microM N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride plus 1 mM ascorbate. Rotenone (4 microM) inhibited respiration by 30% and 2 micro M antimycin A or myxothiazole and 1 mM cyanide inhibited it by 85%. Cyanide-insensitive respiration was partially blocked by 2 mM benzohydroxamic acid, suggesting the presence of an alternative oxidase. Candida albicans mitochondria in situ presented a carboxyatractyloside-insensitive increase of Deltapsi induced by 5 mM ATP and 0.5% BSA, and Deltapsi decrease induced by 10 microM linoleic acid, both suggesting the existence of an uncoupling protein. The presence of this protein was subsequently confirmed by immunodetection and respiration experiments with isolated mitochondria. In conclusion, Candida albicans ATCC 90028 possesses an alternative electron transfer chain and alternative oxidase, both absent in animal cells. These pathways can be exceptional targets for the design of new chemotherapeutic agents. Blockage of these respiratory pathways together with inhibition of the uncoupling protein (another potential target for drug design) could lead to increased production of reactive oxygen species, dysfunction of Candida mitochondria, and possibly to oxidative cell death.
dc.description37
dc.description1455-61
dc.languageeng
dc.relationBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]
dc.relationBraz. J. Med. Biol. Res.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectCandida Albicans
dc.subjectCarrier Proteins
dc.subjectElectron Transport
dc.subjectIon Channels
dc.subjectMembrane Potentials
dc.subjectMembrane Proteins
dc.subjectMitochondrial Proteins
dc.subjectOxidative Phosphorylation
dc.subjectReactive Oxygen Species
dc.subjectSpheroplasts
dc.titleRespiration, Oxidative Phosphorylation, And Uncoupling Protein In Candida Albicans.
dc.typeArtículos de revistas


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