Pharmacological And Histopathological Characterization Of Bothrops Lanceolatus (fer De Lance) Venom-induced Edema.

dc.creatorGuimarães, A Q
dc.creatorCruz-Höfling, M A
dc.creatorFerreira de Araújo, P M
dc.creatorBon, C
dc.creatorLôbo de Araújo, A
dc.date2004-Jul
dc.date2015-11-27T12:58:17Z
dc.date2015-11-27T12:58:17Z
dc.date.accessioned2018-03-29T00:59:16Z
dc.date.available2018-03-29T00:59:16Z
dc.identifierInflammation Research : Official Journal Of The European Histamine Research Society ... [et Al.]. v. 53, n. 7, p. 284-91, 2004-Jul.
dc.identifier1023-3830
dc.identifier10.1007/s00011-004-1258-0
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15241562
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195858
dc.identifier15241562
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296091
dc.descriptionBothrops venoms cause local edema, pain, hemorrhage and necrosis. In this study, we investigated the ability of Bothrops lanceolatus venom to cause edema in rat hind paws and examined the mediators involved. Hind paw edema was induced in male Wister rats by the subplantar injection of venom (12.5-100 microg/paw) in the absence and presence of antagonists. Edema was quantified by hydroplethysmometry at 0.25, 0.5, 2, 4, 6 and 24 h post-injection and was expressed as the percentage increase relative to the contralateral (control) paw. The ability of the venom to release histamine from rat peritoneal mast cells was also assessed. Venom caused dose- and time-dependent edema that was maximal within 15 min but disappeared after 24 h and was accompanied by hemorrhage. Dexamethasone (1 mg/kg, s.c.), methysergide (6 mg/kg, i.p.), HOE 140 (0.6 mg/kg, i.v.) and mepyramine (6 mg/kg, i.p.) significantly ( p < 0.05) reduced edema formation, whereas indomethacin (10 mg/kg, i.p.) was ineffective. Dialysis did not affect venom-induced edema. Venom (1, 10 and 30 microg/ml) caused a concentration-dependent release of histamine (13 +/- 1%, 61.9 +/- 4.6% and 73.6 +/- 2.4%, respectively; n = 5) from rat peritoneal mast cells in vitro. Histological analysis confirmed the presence of edema, hemorrhage and neutrophil infiltration. Pretreating the venom with EDTA partially inhibited the edema and hemorrhage, but did not affect the migration of neutrophils. B. lanceolatus venom produced dose- and time-dependent edema in rat paws. This edema was not dependent on low molecular weight substances in the venom, but was partially dependent on a hemorrhagin and also involved the release of arachidonic acid metabolites, bradykinin, histamine and serotonin.
dc.description53
dc.description284-91
dc.languageeng
dc.relationInflammation Research : Official Journal Of The European Histamine Research Society ... [et Al.]
dc.relationInflamm. Res.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAdrenergic Beta-antagonists
dc.subjectAnimals
dc.subjectAntiemetics
dc.subjectArachidonic Acid
dc.subjectBothrops
dc.subjectBradykinin
dc.subjectCardiovascular Agents
dc.subjectCrotalid Venoms
dc.subjectDexamethasone
dc.subjectDose-response Relationship, Drug
dc.subjectEdema
dc.subjectEdetic Acid
dc.subjectExtremities
dc.subjectHistamine
dc.subjectHistamine H1 Antagonists
dc.subjectIndomethacin
dc.subjectMast Cells
dc.subjectMethysergide
dc.subjectPyrilamine
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSerotonin
dc.subjectTime Factors
dc.subjectVasoconstrictor Agents
dc.titlePharmacological And Histopathological Characterization Of Bothrops Lanceolatus (fer De Lance) Venom-induced Edema.
dc.typeArtículos de revistas


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