Detection Of Bovine Respiratory Syncytial Virus In Experimentally Infected Balb/c Mice.

dc.creatorAlmeida, Renata Servan
dc.creatorDomingues, Helena Gallicchio
dc.creatorCoswig, Lia Treptow
dc.creatorD'Arce, Regina Celia Freitas
dc.creatorde Carvalho, Rodrigo Franco
dc.creatorArns, Clarice Weis
dc.date
dc.date2015-11-27T12:58:05Z
dc.date2015-11-27T12:58:05Z
dc.date.accessioned2018-03-29T00:58:56Z
dc.date.available2018-03-29T00:58:56Z
dc.identifierVeterinary Research. v. 35, n. 2, p. 189-97
dc.identifier0928-4249
dc.identifier10.1051/vetres:2004003
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15099495
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195774
dc.identifier15099495
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1296007
dc.descriptionThe present study used an RT-nested-PCR and an immunohistochemistry assay to detect bovine respiratory syncytial virus in tissues from experimentally infected balb/c mice. As a first step, Chicken Embryo Related (CER) cell monolayers infected with the BRSV-25-BR strain isolated in Brazil were used for antigen production. Then, the infected lung and tracheal tissues of female balb/c mice were collected on 3, 5, 7 and 10 days post-infection and submitted to both techniques. Primers specific to F and G genes that amplify fragments of 481 bp and 371 bp, respectively, were used. The BRSV detection was not successful in all of the animals tested. The genomic fragment of the G gene from the organs of some infected mice on all analyzed post-infection days was amplified. However, in the RT-nested-PCR corresponding to the F gene, it was not possible to observe any amplified fragment. This was probably due to the higher sensitivity of the developed technique to amplify the fragment corresponding to the G gene compared to the F gene. Moreover, only three of the lungs collected five days post-infection were positive by immunohistochemistry. To the author's knowledge, this is the first study reporting bovine respiratory syncytial virus detection in balb/c mice after experimental inoculation.
dc.description35
dc.description189-97
dc.languageeng
dc.relationVeterinary Research
dc.relationVet. Res.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCattle
dc.subjectCattle Diseases
dc.subjectChick Embryo
dc.subjectDna, Viral
dc.subjectFemale
dc.subjectImmunohistochemistry
dc.subjectMice
dc.subjectMice, Inbred Balb C
dc.subjectRespiratory Syncytial Virus Infections
dc.subjectRespiratory Syncytial Virus, Bovine
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectSensitivity And Specificity
dc.subjectSpecific Pathogen-free Organisms
dc.titleDetection Of Bovine Respiratory Syncytial Virus In Experimentally Infected Balb/c Mice.
dc.typeArtículos de revistas


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