Lipid Membrane With Low Proton Permeability.

dc.creatorBiloti, Débora Nakai
dc.creatorSantana, Maria Helena Andrade
dc.creatorPessine, Francisco Benedito Teixeira
dc.date2003-Apr
dc.date2015-11-27T12:52:46Z
dc.date2015-11-27T12:52:46Z
dc.date.accessioned2018-03-29T00:58:41Z
dc.date.available2018-03-29T00:58:41Z
dc.identifierBiochimica Et Biophysica Acta. v. 1611, n. 1-2, p. 1-4, 2003-Apr.
dc.identifier0006-3002
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12659939
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195710
dc.identifier12659939
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295943
dc.descriptionThis work reports the production of a liposomal formulation, having a lipidic membrane with known chemical composition and a low proton permeability, as confirmed by physicochemical characterization of the maintenance of a transmembranic pH gradient. These liposomes consist of DSPC, DSPE-PEG, DSPG and cholesterol, with low internal pH. To verify the low proton permeability of these liposomal bilayers, a study of proton migration according to the fluorescence quenching of 9-aminoacridine (9AA), as well as CPT-11 encapsulation, were used to monitor the acidification of the intravesicular space. Both experiments showed that this liposomal formulation is able to maintain a transmembranic proton gradient.
dc.description1611
dc.description1-4
dc.languageeng
dc.relationBiochimica Et Biophysica Acta
dc.relationBiochim. Biophys. Acta
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAminacrine
dc.subjectCamptothecin
dc.subjectFluorescent Dyes
dc.subjectHydrogen-ion Concentration
dc.subjectLiposomes
dc.subjectMembrane Lipids
dc.subjectPermeability
dc.subjectProtons
dc.subjectSpectrometry, Fluorescence
dc.titleLipid Membrane With Low Proton Permeability.
dc.typeArtículos de revistas


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