Effect Of Phosphate Concentration On The Production Of Dextransucrase By Leuconostoc Mesenteroides Nrrl B512f.

dc.creatorRodrigues, S
dc.creatorLona, L M F
dc.creatorFranco, T T
dc.date2003-Nov
dc.date2015-11-27T12:52:27Z
dc.date2015-11-27T12:52:27Z
dc.date.accessioned2018-03-29T00:58:02Z
dc.date.available2018-03-29T00:58:02Z
dc.identifierBioprocess And Biosystems Engineering. v. 26, n. 1, p. 57-62, 2003-Nov.
dc.identifier1615-7591
dc.identifier10.1007/s00449-003-0330-4
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/14505164
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195536
dc.identifier14505164
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295769
dc.descriptionLeuconostoc mesenteroides NRRL B512F is the main strain used in industrial fermentations to produce dextransucrase and dextran. This process has been studied since the Second World War, when it was used as blood plasma expander. A study about the effect of phosphate concentration on cell propagation in a semicontinuous shake-flask culture is described in this work. Dextransucrase is obtained by fermentation of the Leuconostoc mesenteroides NRRL B512F in the presence of sucrose as substrate, a nitrogen source (corn liquor or yeast extract) and minerals. Phosphate is currently used in order to buffer the culture medium. Cell propagation can be done through a repeated batch culture, where dilution in a fresh medium is made with relatively short periods. The standard medium for dextransucrase production is prepared using 0.1 M of K(2)HPO(4). In this work the level of phosphate was increased to 0.3 M, and an increase on biomass and on the enzyme activity was found when phosphate enriched medium was used. Higher phosphate buffer concentration was also able to keep the pH values above 5.0 during the entire process, avoiding enzyme denaturation.
dc.description26
dc.description57-62
dc.languageeng
dc.relationBioprocess And Biosystems Engineering
dc.relationBioprocess Biosyst Eng
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectBioreactors
dc.subjectCell Culture Techniques
dc.subjectCell Division
dc.subjectComputer Simulation
dc.subjectDextrans
dc.subjectDose-response Relationship, Drug
dc.subjectEnzyme Activation
dc.subjectEnzyme Stability
dc.subjectGlucosyltransferases
dc.subjectHydrogen-ion Concentration
dc.subjectLeuconostoc
dc.subjectModels, Biological
dc.subjectPhosphates
dc.subjectSpecies Specificity
dc.titleEffect Of Phosphate Concentration On The Production Of Dextransucrase By Leuconostoc Mesenteroides Nrrl B512f.
dc.typeArtículos de revistas


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