Iron-responsive Genes Of Phanerochaete Chrysosporium Isolated By Differential Display Reverse Transcription Polymerase Chain Reaction.

dc.creatorAssmann, Eliana Maria
dc.creatorOttoboni, Laura Maria Mariscal
dc.creatorFerraz, André
dc.creatorRodríguez, Jaime
dc.creatorDe Mello, Maricilda Palandi
dc.date2003-Sep
dc.date2015-11-27T12:52:21Z
dc.date2015-11-27T12:52:21Z
dc.date.accessioned2018-03-29T00:57:50Z
dc.date.available2018-03-29T00:57:50Z
dc.identifierEnvironmental Microbiology. v. 5, n. 9, p. 777-86, 2003-Sep.
dc.identifier1462-2912
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12919413
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195487
dc.identifier12919413
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295720
dc.descriptionWhite-rot fungus Phanerochaete chrysosporium, a ligninolytic basidiomycete, was studied to identify iron-responsive genes. Using the differential display reverse transcription PCR technique (DDRT-PCR), a total of 97 differentially expressed cDNA fragments were identified by comparing band intensities among fingerprints obtained from mycelia cultivated in iron-deficient and iron-replete media. Transcripts induced under iron-starvation exhibited homologies to: a modular polyketide synthase, a TonB protein, a probable transmembrane protein, a putative ABC transporter permease and a HSP70-related heat-shock protein. Modular polyketide synthase and TonB proteins are normally expressed under iron-starvation and are known to be involved in biosynthesis and transport of siderophores respectively. Also, a deduced protein with 96% similarity to a precursor of the well-known P. chrysosporium lignin peroxidase was identified under iron-deficiency. Two DDRT-PCR products confirmed their iron-induced expression. One was homologue to the CNOT3, which is a global regulator of RNA polymerase II transcription and has been implicated in multiple roles in the control of mRNA metabolism. The other was similar to the Schizosaccharomyces pombe putative proteasome maturation factor upm1. In conclusion, the majority of iron-responsive P. chrysosporium transcripts isolated in the DDRT-PCR encode proteins involved in iron acquisition, especially members of biosynthesis and transport of iron chelators.
dc.description5
dc.description777-86
dc.languageeng
dc.relationEnvironmental Microbiology
dc.relationEnviron. Microbiol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectBlotting, Northern
dc.subjectElectrophoresis, Agar Gel
dc.subjectElectrophoresis, Polyacrylamide Gel
dc.subjectFungal Proteins
dc.subjectGene Expression Profiling
dc.subjectGene Expression Regulation, Fungal
dc.subjectGenes, Fungal
dc.subjectHsp70 Heat-shock Proteins
dc.subjectIron
dc.subjectIron-regulatory Proteins
dc.subjectMembrane Proteins
dc.subjectMembrane Transport Proteins
dc.subjectMolecular Sequence Data
dc.subjectMultienzyme Complexes
dc.subjectPeroxidases
dc.subjectPhanerochaete
dc.subjectRna, Fungal
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectSequence Analysis, Dna
dc.subjectSequence Homology, Nucleic Acid
dc.subjectSiderophores
dc.subjectTranscription, Genetic
dc.titleIron-responsive Genes Of Phanerochaete Chrysosporium Isolated By Differential Display Reverse Transcription Polymerase Chain Reaction.
dc.typeArtículos de revistas


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