Structural And Biological Characterization Of A Crotapotin Isoform Isolated From Crotalus Durissus Cascavella Venom.

dc.creatorde Oliveira, Daniela G
dc.creatorToyama, Marcos H
dc.creatorMartins, Alice M C
dc.creatorHavt, Alexandre
dc.creatorNobre, Arlândia C L
dc.creatorMarangoni, Sergio
dc.creatorCâmara, Paula R
dc.creatorAntunes, Edson
dc.creatorde Nucci, Gilberto
dc.creatorBeliam, Luis O S
dc.creatorFonteles, Manassés C
dc.creatorMonteiro, Helena S A
dc.date2003-Jul
dc.date2015-11-27T12:52:20Z
dc.date2015-11-27T12:52:20Z
dc.date.accessioned2018-03-29T00:57:48Z
dc.date.available2018-03-29T00:57:48Z
dc.identifierToxicon : Official Journal Of The International Society On Toxinology. v. 42, n. 1, p. 53-62, 2003-Jul.
dc.identifier0041-0101
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12893061
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195475
dc.identifier12893061
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295708
dc.descriptionEnvenoming by Crotalus durissus subspecies leads to coagulation disorders, myotoxicity, neurotoxicity and acute renal failure. The most serious systemic alteration and primary cause of death after snakebite is acute renal failure. In this work, we isolated crotapotin, an acid component (Crtp) of crotoxin from Crotalus durissus cascavella venom and we investigated its bactericidal and pro-inflammatory activities as well as its renal effects in rat isolated perfused kidneys. Crtp was bactericidal to the Gram-negative species Xanthomonas axonopodis pv. passiflorae, but was less effective against the Gram-positive Claribacteri ssp, probably because of differences in the cell wall composition. Crtp showed a high amino acid sequence homology with other Crtps described in the literature (around of 90%) and its A and B chains had high conserved regions corresponding to the calcium-binding loop, catalytic site and helix 3 of PLA2. The Crtp showed moderate pro-inflammatory activity and increased significantly the inflammation evoked by PLA2 when co-injected or co-incubated with PLA2. The renal parameters evaluated included the perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF), glomerular filtration rate (GFR) and percent of sodium tubular transport (%TNa+). Crotapotin (5 microg/ml) significantly increased the PP and RVR, whereas the GFR, UF and %TNa+ were unaffected. These results suggest that crotoxin is the main venom component responsible for nephrotoxicity and crotapotin contributes little to this phenomenom. The biological and bactericidal actions of Crtp also suggest that this protein may have functions other than simply acting as a chaperone for PLA2.
dc.description42
dc.description53-62
dc.languageeng
dc.relationToxicon : Official Journal Of The International Society On Toxinology
dc.relationToxicon
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectActinomycetales
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectChromatography, High Pressure Liquid
dc.subjectCrotalid Venoms
dc.subjectCrotoxin
dc.subjectDose-response Relationship, Drug
dc.subjectDrug Synergism
dc.subjectEdema
dc.subjectHindlimb
dc.subjectKidney
dc.subjectMale
dc.subjectMolecular Sequence Data
dc.subjectPerfusion
dc.subjectPhospholipases A
dc.subjectPhospholipases A2
dc.subjectProtein Isoforms
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSequence Alignment
dc.subjectSkin
dc.subjectXanthomonas
dc.titleStructural And Biological Characterization Of A Crotapotin Isoform Isolated From Crotalus Durissus Cascavella Venom.
dc.typeArtículos de revistas


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