Cholesteryl Ester Transfer Protein Expression Is Down-regulated In Hyperinsulinemic Transgenic Mice.

dc.creatorBerti, J A
dc.creatorCasquero, A C
dc.creatorPatricio, P R
dc.creatorBighetti, E J B
dc.creatorCarneiro, E M
dc.creatorBoschero, A C
dc.creatorOliveira, H C F
dc.date2003-Oct
dc.date2015-11-27T12:52:17Z
dc.date2015-11-27T12:52:17Z
dc.date.accessioned2018-03-29T00:57:42Z
dc.date.available2018-03-29T00:57:42Z
dc.identifierJournal Of Lipid Research. v. 44, n. 10, p. 1870-6, 2003-Oct.
dc.identifier0022-2275
dc.identifier10.1194/jlr.M300036-JLR200
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12867535
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195451
dc.identifier12867535
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295684
dc.descriptionCholesteryl ester transfer protein (CETP) mediates cholesteryl ester (CE) and triglyceride redistribution among plasma lipoproteins. In this work, we investigated whether varying levels of insulin regulate the CETP expression in vivo. Insulin deficiency [streptozotocin (STZ) injection], and hyperinsulinemia (insulin injections, 14 days) were induced in transgenic mice expressing a human CETP minigene flanked by its natural regulatory sequences. Glucose supplementation was provided to the hyperinsulinemic group (INS+GLUC) and to an extra group of mice (GLUC). In the STZ group, endogenous CE transfer rate, plasma CETP, and hepatic CETP mRNA levels were enhanced 3.0-, 1.5-, and 2.5-fold, respectively, as compared with controls. Insulin replacement in STZ mice normalized their glycemia and liver mRNA levels. Higher plasma CETP levels were observed in GLUC mice, which were decreased in INS+GLUC mice. Hepatic CETP mRNA was not altered in GLUC mice and was reduced by one-third in INS+GLUC mice. These results show that: 1) STZ treatment increases CETP plasma levels and liver mRNA expression; 2) diet glucose supplementation increases plasma CETP levels but does not change liver mRNA abundance; and 3) daily insulin injections blunt the glucose-stimulated CETP expression by reducing its liver mRNA levels. These data suggest that insulin down-regulates CETP gene expression.
dc.description44
dc.description1870-6
dc.languageeng
dc.relationJournal Of Lipid Research
dc.relationJ. Lipid Res.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBody Composition
dc.subjectCarrier Proteins
dc.subjectCholesterol Ester Transfer Proteins
dc.subjectCholesterol Esters
dc.subjectDown-regulation
dc.subjectGene Expression Regulation
dc.subjectGlucose
dc.subjectGlycoproteins
dc.subjectHumans
dc.subjectHyperinsulinism
dc.subjectInsulin
dc.subjectLipoproteins
dc.subjectLiver
dc.subjectMice
dc.subjectMice, Transgenic
dc.subjectRna, Messenger
dc.titleCholesteryl Ester Transfer Protein Expression Is Down-regulated In Hyperinsulinemic Transgenic Mice.
dc.typeArtículos de revistas


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