Intracellular Vacuolation Induced By Culture Filtrates Of Plesiomonas Shigelloides Isolated From Environmental Sources.

dc.creatorFalcón, R
dc.creatorCarbonell, G V
dc.creatorFigueredo, P M S
dc.creatorButião, F
dc.creatorSaridakis, H O
dc.creatorPelayo, J S
dc.creatorYano, T
dc.date2003
dc.date2015-11-27T12:52:16Z
dc.date2015-11-27T12:52:16Z
dc.date.accessioned2018-03-29T00:57:41Z
dc.date.available2018-03-29T00:57:41Z
dc.identifierJournal Of Applied Microbiology. v. 95, n. 2, p. 273-8, 2003.
dc.identifier1364-5072
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12859758
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195446
dc.identifier12859758
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295679
dc.descriptionPotential virulence factors produced by culture filtrates of Plesiomonas shigelloides isolated from water were investigated. Culture filtrates of P. shigelloides strains were assayed for cytotoxic activity in CHO (Chinese hamster ovary), Vero (African green monkey kidney), HeLa (human cervix), HT29 (human epithelial intestinal) and SK6 (swine epithelial kidney) cells. Microscopic analyses revealed intensive cytoplasmic vacuolation including cell rounding and swelling, with gradual destruction of the monolayer in filtrate-treated cells. Neutral red assays showed that CHO, HeLa and Vero cells were the most sensitive to the vacuolating activity, which was evident within 30 min of culture filtrate exposure. This activity was inactived by heating at 56 degrees C for 15 min and partially neutralized by antiserum to the cytotoxin of Aeromonas hydrophila. All P. shigelloides strains had a cell-associated haemolysin in the agar plate assay. Three isolates were found to produce a cell-free haemolytic activity at 37 degrees C. In the suckling mouse test, two P. shigelloides culture supernatants were positive for enterotoxic activity. P. shigelloides culture filtrates isolated from aquatic environment cause intracellular vacuolation on mammalian cells, and produce haemolytic and enterotoxic activities. This work revealed the presence of putative virulence factors that could be associated with human infections involving Plesiomonas strains.
dc.description95
dc.description273-8
dc.languageeng
dc.relationJournal Of Applied Microbiology
dc.relationJ. Appl. Microbiol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCell Line
dc.subjectCell Survival
dc.subjectCulture Media, Conditioned
dc.subjectCytotoxins
dc.subjectHemolysin Proteins
dc.subjectHemolysis
dc.subjectHumans
dc.subjectPlesiomonas
dc.subjectVacuoles
dc.subjectVirulence
dc.subjectWater Microbiology
dc.titleIntracellular Vacuolation Induced By Culture Filtrates Of Plesiomonas Shigelloides Isolated From Environmental Sources.
dc.typeArtículos de revistas


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