dc.creatorCesquini, M
dc.creatorTorsoni, M A
dc.creatorStoppa, G R
dc.creatorOgo, S H
dc.date
dc.date2015-11-27T12:52:13Z
dc.date2015-11-27T12:52:13Z
dc.date.accessioned2018-03-29T00:57:34Z
dc.date.available2018-03-29T00:57:34Z
dc.identifierBiomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie. v. 57, n. 3-4, p. 124-9
dc.identifier0753-3322
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12818473
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195421
dc.identifier12818473
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295654
dc.descriptionSickle cell anemia is a genetic disease characterized byan increase in generation of reactive oxygen species, abnormal iron release and low antioxidant activity which can lead to cell injury. Several therapies have been used to decrease the oxidative damage in these patients. In this study, we investigated the effect of flavonoids (quercetin and rutin) on the oxidation of red blood cells (RBC) from sickle cell anemia patients following exposure of the cells to tert-butyl hydroperoxide (t-BOOH). Quercetin provided greater protection against Hb oxidation, the binding of Hb to membrane and lipid peroxidation than did rutin. Quercetin (150 microM) reduced Hb oxidation by 30% and increased the level of oxyHb from 17.5 to 29 microM. Rutin prevented Hb oxidation only at concentrations higher than 200 microM and did not prevent the binding of Hb to RBC membrane. These distinct effects of the flavonoids probably reflect their structural characteristics. Thus, quercetin, which possesses a suitable structure for free-radical scavenging and ion quelation, was a more effective antioxidant than rutin. The presence of rutinose at position C(3) in rutin may impair its antioxidant effect. The presence of ascorbic acid enhanced the protective effect of quercetin and rutin against oxidative stress in sickle Hb and lipid peroxidation. This synergistic action helped to maintain a constant supply of flavonoids and thus, rescue the cells from the injury caused by free radicals and iron ions.
dc.description57
dc.description124-9
dc.languageeng
dc.relationBiomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
dc.relationBiomed. Pharmacother.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnemia, Sickle Cell
dc.subjectAntioxidants
dc.subjectAntisickling Agents
dc.subjectBlood Transfusion
dc.subjectErythrocytes
dc.subjectFlavonoids
dc.subjectHemoglobins
dc.subjectHumans
dc.subjectIn Vitro Techniques
dc.subjectLipid Peroxidation
dc.subjectOxidants
dc.subjectOxidation-reduction
dc.subjectOxidative Stress
dc.subjectQuercetin
dc.subjectRutin
dc.subjectTert-butylhydroperoxide
dc.titleT-booh-induced Oxidative Damage In Sickle Red Blood Cells And The Role Of Flavonoids.
dc.typeArtículos de revistas


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